干旱区生态环境知识资源中心(Arid): Biotechnological potential of bacteria isolated from cattle environments of desert soils in Sonora Mexico

Arid

DOI	10.1007/s11274-018-2574-9
	Biotechnological potential of bacteria isolated from cattle environments of desert soils in Sonora Mexico
	Baqueiro-Pena, Itzamna 1; Asaff-Torres, Ali 1; Kirchmayr, Manuel R.2; Valenzuela-Soto, Elisa M.1; Zamora, Arturo 1
通讯作者	Baqueiro-Pena, Itzamna
来源期刊	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN	0959-3993
EISSN	1573-0972
出版年	2019
卷号	35 期号:1
英文摘要	The aim of this research was to study the hydrolytic potential of bacteria isolated from cattle environments of two desert soils in one of the driest and hottest zones in America. A total of 26 points were sampled, 144 strains were isolated, and 50 strains were selected for the characterization of esterase, lipase, protease, and amylase activities and for 16S rRNA identification. Strains of the Bacillus, Pseudomonas, Acinetobacter, Enterobacter, Providencia, Escherichia, and Pantoea genera were identified. Comparisons of the proteolytic activity of the secretome from 14 strains (Bacillus n=7, Escherichia n=2; Providencia, Pseudomonas, Enterobacter, Pantoea and Acinetobacter n=1) were performed. Four strains of Bacillus showed the highest proteolytic activity. These strains were characterized through a comparative analysis of pH and temperature as well as the effects of salt concentration on protease activity. Maximum proteolytic activity occurred in the range of pH 7-9 and temperatures between 50 and 70 degrees C for B. subtilis WD01, B. tequilensis WS11, B. tequilensis WS13, and B. tequilensis WS14. At a 20% NaCl concentration, the proteolytic activity retained was 71.4%, 65%, and 79.8% for WD01, WS11, and WS13, respectively; the activity of strain WS14 increased with 45% NaCl. Protease production by B. tequilensis WS14 with wheat, fish, and bone flours as low-cost substrates showed no differences between bone and fish flours and showed a decrease in protease production with wheat flour. The proteolytic activity in flour extracts with 20% NaCl was 82%, 75.61% and 38.04% for fish, bone and wheat flours, respectively. Data obtained in this work allow us to propose that strains isolated from environments with extreme conditions have a biotechnological potential.
英文关键词	Protease Microbial bioprospecting Soil Growth promoting
类型	Article
语种	英语
国家	Mexico
收录类别	SCI-E
WOS记录号	WOS:000453326300002
WOS关键词	ALKALINE PROTEASE ; PROBIOTIC BACTERIA ; BACILLUS-SUBTILIS ; PURIFICATION ; DIVERSITY ; ACINETOBACTER ; RESISTANCE
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/219344
作者单位	1.Ctr Invest Alimentac & Desarrollo AC, Carr Gustavo Enrique Astiazaran 46 AP 1735, Hermosillo 83000, Sonora, Mexico; 2.CIATEJ Ctr Invest & Asistencia Tecnol & Diseno Es, Camino Arenero 1227, Zapopan 45019, Jal, Mexico
推荐引用方式 GB/T 7714	Baqueiro-Pena, Itzamna,Asaff-Torres, Ali,Kirchmayr, Manuel R.,et al. Biotechnological potential of bacteria isolated from cattle environments of desert soils in Sonora Mexico[J],2019,35(1).
APA	Baqueiro-Pena, Itzamna,Asaff-Torres, Ali,Kirchmayr, Manuel R.,Valenzuela-Soto, Elisa M.,&Zamora, Arturo.(2019).Biotechnological potential of bacteria isolated from cattle environments of desert soils in Sonora Mexico.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,35(1).
MLA	Baqueiro-Pena, Itzamna,et al."Biotechnological potential of bacteria isolated from cattle environments of desert soils in Sonora Mexico".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 35.1(2019).