干旱区生态环境知识资源中心(Arid): Epidemiology of tick-borne pathogens in the semi-arid and the arid agro-ecological zones of Punjab province, Pakistan

Arid

DOI	10.1111/tbed.13059
	Epidemiology of tick-borne pathogens in the semi-arid and the arid agro-ecological zones of Punjab province, Pakistan
	Rehman, Abdul 1,2,3; Conraths, Franz J.2; Sauter-Louis, Carola 2; Kruecken, Juergen 1; Nijhof, Ard M.1
通讯作者	Nijhof, Ard M.
来源期刊	TRANSBOUNDARY AND EMERGING DISEASES
ISSN	1865-1674
EISSN	1865-1682
出版年	2019
卷号	66 期号:1 页码:526-536
英文摘要	Tick-borne diseases (TBDs) have a large impact on animal health and the livelihood of livestock owners, particularly in developing countries. Although climatic and ecological conditions in Pakistan may favour the transmission of tick-borne pathogens (TBPs), only a few systematic studies have been carried out on TBPs and the diseases that they cause in this country. To improve our understanding of the distribution of TBPs, 3,807 ticks were collected from ruminants (n = 369) on 108 livestock farms (semi-arid zone = 36, arid zone = 72) in Punjab Province. After morphological identification ticks were pooled into 405 pools (Hyalomma anatolicum = 300, Rhipicephalus microplus = 89, Hyalomma dromedarii = 9, Rhipicephalus turanicus = 7) based on their species, locality of collection, and the host. DNA from each pool was screened by a Reverse Line Blot (RLB) hybridization assay for the presence of Anaplasma, Ehrlichia, Rickettsia, Babesia, and Theileria species. DNA from at least one TBP was found in 142 (35.1%) pools. Among the positive pools, 91 (64.1%) had a mixed infection with two or more TBPs, whereas 51 (35.9%) pools were infected with a single TBP. The detected pathogens not only included species that were known to be endemic in Pakistan, such as Theileria annulata (6.7%), Theileria orientalis (3.5%), Anaplasma marginale (5.7%), Anaplasma centrale (2.7%), Anaplasma ovis (1.5%), Babesia bigemina (0.7%), and Babesia bovis (0.2%), but also several TBPs that had not been reported to occur in Pakistan before. This included Ehrlichia minasensis (3.2%), an Anaplasma platys-like organism (1.2%), Babesia occultans (0.2%), and Rickettsia massiliae (0.2%), as well as two previously uncharacterized species: Ehrlichia sp. Multan (18.0%) and Anaplasma sp. (BL099-6) (2.22%). The pathogenicity of these novel species remains to be examined. This study shows that a much broader spectrum of TBPs is present in Pakistan than previously thought, including several zoonotic pathogens.
英文关键词	agro-ecological zones Pakistan Reverse Line Blot hybridization assay tick-borne pathogens ticks
类型	Article
语种	英语
国家	Germany ; Pakistan
收录类别	SCI-E
WOS记录号	WOS:000456344900057
WOS关键词	SPOTTED-FEVER GROUP ; MULTIPLE SEQUENCE ALIGNMENT ; THEILERIA-ORIENTALIS ; RICKETTSIA-MASSILIAE ; MOLECULAR CHARACTERIZATION ; ANAPLASMA-MARGINALE ; BOOPHILUS-MICROPLUS ; WEST-PAKISTAN ; RISK-FACTORS ; PREVALENCE
WOS类目	Infectious Diseases ; Veterinary Sciences
WOS研究方向	Infectious Diseases ; Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/219113
作者单位	1.Free Univ Berlin, Inst Parasitol & Trop Vet Med, Berlin, Germany; 2.Inst Epidemiol, Friedrich Loeffler Inst, Greifswald, Germany; 3.Univ Vet & Anim Sci, Dept Epidemiol & Publ Hlth, Lahore, Pakistan
推荐引用方式 GB/T 7714	Rehman, Abdul,Conraths, Franz J.,Sauter-Louis, Carola,et al. Epidemiology of tick-borne pathogens in the semi-arid and the arid agro-ecological zones of Punjab province, Pakistan[J],2019,66(1):526-536.
APA	Rehman, Abdul,Conraths, Franz J.,Sauter-Louis, Carola,Kruecken, Juergen,&Nijhof, Ard M..(2019).Epidemiology of tick-borne pathogens in the semi-arid and the arid agro-ecological zones of Punjab province, Pakistan.TRANSBOUNDARY AND EMERGING DISEASES,66(1),526-536.
MLA	Rehman, Abdul,et al."Epidemiology of tick-borne pathogens in the semi-arid and the arid agro-ecological zones of Punjab province, Pakistan".TRANSBOUNDARY AND EMERGING DISEASES 66.1(2019):526-536.