干旱区生态环境知识资源中心(Arid): Control of beta-sitosterol biosynthesis under light and watering in desert plant Calotropis procera

Arid

DOI	10.1016/j.steroids.2018.11.003
	Control of beta-sitosterol biosynthesis under light and watering in desert plant Calotropis procera
	Ramadan, Ahmed M.1,2; Azeiz, Ahmed Abdel 3; Baabad, Saeed 1; Hassanein, Sameh 3,4; Gadalla, Nour O.5,6; Hassan, Sabah 1,7; Algandaby, Mardi 1; Bakr, Salwa 8,9; Khan, Thana 1; Abouseadaa, Heba H.10; Ali, Hani Mohammed 1; Al-Ghamdi, Areej 11; Osman, Gamal 12,13; Edris, Sherif 1,7; Eissa, Hala 2,3; Bahieldin, Ahmed 1,7
通讯作者	Ramadan, Ahmed M. ; Osman, Gamal
来源期刊	STEROIDS
ISSN	0039-128X
EISSN	1878-5867
出版年	2019
卷号	141 页码:1-8
英文摘要	Most scientific studies on Calotropis procera refer to the plant as an important source of pharmaceutical compounds and its valuable benefits in medicine. One of the most important substances in this plant is the potential immunostimulant beta-sitosterol (BS) that acts in improving human health. This study focused on the effects of lighting before and after irrigation on the BS accumulation pathway namely steroid biosynthesis. Studying the enzymes in BS biosynthetic pathway indicated the upregulation at dawn and predusk of the SMT2 and SMO2 genes encoding sterol methyltransferase 2 and methylsterol monooxygenase, two key enzymes in BS accumulation in C. procera. The results almost indicated no regulation at the different time points of the CYP710A gene encoding sterol 22-desaturase, an enzyme that acts in depleting beta-sitosterol towards the biosynthesis of stigmasterol. RNA-Seq data was validated via quantitative RT-PCR and results were positive. The data of ultra-performance liquid chromatography-tandem mass spectrometry analysis with regard to BS accumulation also aligned with those of RNA-Seq analysis. We focused on the effects of light before and after watering on BS accumulation in C. procera. Our results show that BS accumulation is high at dawn in both dehydrated and well-watered condition. While, the BS was dramatically decrease at midday in well-watered plants. This increase/decrease in BS content is correlated with rates of expression of SMT 2 gene. This gene is a key convertor between the different branches in the cardiac glycoside biosynthesis. Accordingly, it could be suggested that BS (or one of the descendent product) may play an important role in C. procera tolerance to drought/light intensity conditions.
英文关键词	beta-Sitosterol Light Watering Calotropis procera RNA-Seq analysis
类型	Article
语种	英语
国家	Saudi Arabia ; Egypt
收录类别	SCI-E
WOS记录号	WOS:000456638900001
WOS关键词	TRANSCRIPTOME ; PHYTOSTEROLS ; TOLERANCE ; GLYCOSIDE ; STEROLS ; PATHWAY ; ROOTS
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218937
作者单位	1.King Abdulaziz Univ, Fac Sci, Biol Sci Dept, POB 80141, Jeddah 21589, Saudi Arabia; 2.ARC, AGERI, Plant Mol Biol Dept, Giza, Egypt; 3.MUST, Coll Biotechnol, 6Th October City, Egypt; 4.ARC, AGERI, Bioinformat Dept, Giza, Egypt; 5.King Abdulaziz Univ, Fac Meteorol Environm & Arid Land Agr, Dept Arid Land Agr, Jeddah, Saudi Arabia; 6.Natl Res Ctr, Genet Engn & Biotechnol Div, Genet & Cytol Dept, Dokki, Egypt; 7.Ain Shams Univ, Dept Genet, Fac Agr, Cairo, Egypt; 8.Fayoum Univ, Coll Med, Dept Clin Pathol, Hematol, Al Fayyum, Egypt; 9.Princess Nourah Bint Abdulrahman Univ, Coll Med, Riyadh, Saudi Arabia; 10.Ain Shams Univ, Dept Bot, Fac Sci, Cairo, Egypt; 11.Jeddah Univ, Fac Sci, Phys Dept, Jeddah, Saudi Arabia; 12.Umm Al Qura Univ, Fac Appl Sci, Dept Biol, Mecca, Saudi Arabia; 13.ARC, AGERI, Dept Microbial Genet, Giza, Egypt
推荐引用方式 GB/T 7714	Ramadan, Ahmed M.,Azeiz, Ahmed Abdel,Baabad, Saeed,et al. Control of beta-sitosterol biosynthesis under light and watering in desert plant Calotropis procera[J],2019,141:1-8.
APA	Ramadan, Ahmed M..,Azeiz, Ahmed Abdel.,Baabad, Saeed.,Hassanein, Sameh.,Gadalla, Nour O..,...&Bahieldin, Ahmed.(2019).Control of beta-sitosterol biosynthesis under light and watering in desert plant Calotropis procera.STEROIDS,141,1-8.
MLA	Ramadan, Ahmed M.,et al."Control of beta-sitosterol biosynthesis under light and watering in desert plant Calotropis procera".STEROIDS 141(2019):1-8.