干旱区生态环境知识资源中心(Arid): A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum

Arid

DOI	10.1016/j.saa.2018.09.036
	A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum
	Mishra, Kumud Bandhu 1,2; Vitek, Petr 2; Bartak, Milos 1
通讯作者	Mishra, Kumud Bandhu
来源期刊	SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
ISSN	1386-1425
EISSN	1873-3557
出版年	2019
卷号	208 页码:13-23
英文摘要	Lichens are successful colonizers in extreme environments worldwide, and they are considered to have played an important role during the evolution of life. Here, we have used a correlative approach, combining three optical signals (chlorophyll a fluorescence (ChlF), reflectance, and Raman spectra), to monitor hydration induced changes in photosynthetic properties of an Antarctic chlorolichen Dermatocarpon polyphyllizum. We measured these three signals from this lichen at different stages (after 4 h, 24 h, and 48 h) of hydration, and compared the data obtained from this lichen in dry state as well as in different hydrated state. We found that dry state of this lichen has: (1) no variable ChlF, (2) high reflectance, with no red-edge and almost zero photochemical reflectance index (PRI), and (3) low-intensity Raman bands of their carotenoids. Furthermore, 4 h of hydration, increased its relative water content (RWC) by 93%, showed red-edge in reflectance spectra, and changed the maximum quantum yield of NH photochemistry (F-v/F-m) from 0 to 0.57 +/- 0.01. We found that reflectance indices, normalized difference index (NDVI) and PRI, significantly differed between brown and black/green surface areas, at all hydration stages; whereas, a shift in the Raman v(l) (C=C) band, between brown and black/green surface areas, occurred in 24 h or 48 h hydrated samples. These data indicate that hydration shortly (within 4 h) activated functions of photosynthetic apparatus, and the de novo synthesis of carotenoids occured in 24 h or 48 h. Furthermore, exposure to high irradiance (2000 mu mol photons m(-2) s(-1)), in 48 h hydrated lichen, significantly reduced F-v/F-m (signifies photoinhibition) and increased PRI (represents changes in xanthophyll pigments). We conclude that the implication of such a correlative approach is highly useful for understanding survival and protective mechanisms on extremophile photosynthetic organisms. (C) 2018 Published by Elsevier B.V.
英文关键词	Carotenoids Chlorophyll a fluorescence transient Extremophile organisms Optical signal Raman mapping Reflectance spectra
类型	Article
语种	英语
国家	Czech Republic
收录类别	SCI-E
WOS记录号	WOS:000449900700003
WOS关键词	BLUE-GREEN-ALGAE ; WATER-VAPOR UPTAKE ; SPECTRAL SIGNATURES ; PHOTOSYNTHESIS ; DESERT ; DESICCATION ; THALLI ; PHOTOPROTECTION ; PHOTOINHIBITION ; CYANOBACTERIA
WOS类目	Spectroscopy
WOS研究方向	Spectroscopy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218925
作者单位	1.Masaryk Univ, Fac Sci, Dept Expt Biol, Kamenice 5, Brno 62500, Czech Republic; 2.Czech Acad Sci, Global Change Res Inst, Belidla 986-4a, Brno 60300, Czech Republic
推荐引用方式 GB/T 7714	Mishra, Kumud Bandhu,Vitek, Petr,Bartak, Milos. A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum[J],2019,208:13-23.
APA	Mishra, Kumud Bandhu,Vitek, Petr,&Bartak, Milos.(2019).A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,208,13-23.
MLA	Mishra, Kumud Bandhu,et al."A correlative approach, combining chlorophyll a fluorescence, reflectance, and Raman spectroscopy, for monitoring hydration induced changes in Antarctic lichen Dermatocarpon polyphyllizum".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 208(2019):13-23.