干旱区生态环境知识资源中心(Arid): Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi

Arid

DOI	10.1038/s41598-019-47357-4
	Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi
	Ghodke, Amol Bharat 1; Good, Robert Trygve 1; Golz, John F.1; Russell, Derek A.2; Edwards, Dwain 3; Robin, Charles 1
通讯作者	Robin, Charles
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2019
卷号	9
英文摘要	Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with or without transfection reagents. dsRNA produced in planta also failed to induce significant RNAi in M. persicae. Transcriptome analyses of the midgut suggested other potential targets including the Ferritin heavy chain transcripts, but they also could not be knocked down with dsRNA. Here we show that dsRNA is rapidly degraded by midgut secretions of Myzus persicae. Analysis of the transcriptome of the M. persicae midgut revealed that an ortholog of RNases from other insects was abundant.
类型	Article
语种	英语
国家	Australia
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000480680800012
WOS关键词	DOUBLE-STRANDED-RNA ; PLANT-MEDIATED RNAI ; ACYRTHOSIPHON-PISUM ; DESERT LOCUST ; PEA APHID ; NUCLEASE ACTIVITY ; INSECT PEST ; INTERFERENCE ; GENE ; GUT
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
来源机构	Commonwealth Scientific and Industrial Research Organisation
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218803
作者单位	1.Univ Melbourne, Sch BioSci, Melbourne, Vic, Australia; 2.Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic, Australia; 3.CSIRO, Land & Water, Perth, WA, Australia
推荐引用方式 GB/T 7714	Ghodke, Amol Bharat,Good, Robert Trygve,Golz, John F.,et al. Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi[J]. Commonwealth Scientific and Industrial Research Organisation,2019,9.
APA	Ghodke, Amol Bharat,Good, Robert Trygve,Golz, John F.,Russell, Derek A.,Edwards, Dwain,&Robin, Charles.(2019).Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi.SCIENTIFIC REPORTS,9.
MLA	Ghodke, Amol Bharat,et al."Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi".SCIENTIFIC REPORTS 9(2019).