干旱区生态环境知识资源中心(Arid): Juvenile Hormone receptor Met is essential for ovarian maturation in the Desert Locust, Schistocerca gregaria

Arid

DOI	10.1038/s41598-019-47253-x
	Juvenile Hormone receptor Met is essential for ovarian maturation in the Desert Locust, Schistocerca gregaria
	Gijbels, Marijke 1; Lenaerts, Cynthia 1; Vanden Broeck, Jozef 1; Marchal, Elisabeth 1,2
通讯作者	Vanden Broeck, Jozef ; Marchal, Elisabeth
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2019
卷号	9
英文摘要	Juvenile hormones (JH) are key endocrine regulators produced by the corpora allata (CA) of insects. Together with ecdysteroids, as well as nutritional cues, JH coordinates different aspects of insect postembryonic development and reproduction. The function of the recently characterized JH receptor, Methoprene-tolerant (Met), appears to be conserved in different processes regulated by JH. However, its functional interactions with other hormonal signalling pathways seem highly dependent on the feeding habits and on the developmental and reproductive strategies employed by the insect species investigated. Here we report on the effects of RNA interference (RNAi) mediated SgMet knockdown during the first gonadotrophic cycle in female desert locusts (Schistocerca gregaria). This voracious, phytophagous pest species can form migrating swarms that devastate field crops and harvests in several of the world's poorest countries. A better knowledge of the JH signalling pathway may contribute to the development of novel, more target-specific insecticides to combat this very harmful swarming pest. Using RNAi, we show that the JH receptor Met is essential for ovarian maturation, vitellogenesis and associated ecdysteroid biosynthesis in adult female S. gregaria. Interestingly, knockdown of SgMet also resulted in a significant decrease of insulin-related peptide (SgIRP) and increase of neuroparsin (SgNP) 3 and 4 transcript levels in the fat body, illustrating the existence of an intricate regulatory interplay between different hormonal factors. In addition, SgMet knockdown in females resulted in delayed display of copulation behaviour with virgin males, when compared with dsGFP injected control animals. Moreover, we observed an incapacity of adult dsSgMet injected female locusts to oviposit during the time of the experimental setup. As such, SgMet is an essential gene playing crucial roles in the endocrine communication necessary for successful reproduction of the desert locust.
类型	Article
语种	英语
国家	Belgium
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000477015300022
WOS关键词	RED FLOUR BEETLE ; METHOPRENE-TOLERANT ; FOLLICLE CELLS ; ENZYME-IMMUNOASSAY ; SIGNALING PATHWAY ; RNA INTERFERENCE ; LINDEN BUG ; FAT-BODY ; INSULIN ; GENE
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218802
作者单位	1.Katholieke Univ Leuven, Res Grp Mol Dev Physiol & Signal Transduct, Zool Inst, Naamsestr 59 Box 2465, B-3000 Leuven, Belgium; 2.IMEC, Kapeldreef 75, B-3001 Leuven, Belgium
推荐引用方式 GB/T 7714	Gijbels, Marijke,Lenaerts, Cynthia,Vanden Broeck, Jozef,et al. Juvenile Hormone receptor Met is essential for ovarian maturation in the Desert Locust, Schistocerca gregaria[J],2019,9.
APA	Gijbels, Marijke,Lenaerts, Cynthia,Vanden Broeck, Jozef,&Marchal, Elisabeth.(2019).Juvenile Hormone receptor Met is essential for ovarian maturation in the Desert Locust, Schistocerca gregaria.SCIENTIFIC REPORTS,9.
MLA	Gijbels, Marijke,et al."Juvenile Hormone receptor Met is essential for ovarian maturation in the Desert Locust, Schistocerca gregaria".SCIENTIFIC REPORTS 9(2019).