干旱区生态环境知识资源中心(Arid): Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss

Arid

DOI	10.1038/s41598-018-38094-1
	Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss
	Ashok, Anup 1; Doriya, Kruthi 1; Rao, Jyothi Vithal 1; Qureshi, Asif 2; Tiwari, Anoop Kumar 3; Kumar, Devarai Santhosh 1
通讯作者	Kumar, Devarai Santhosh
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2019
卷号	9
英文摘要	L-Asparaginase (L-asparagine aminohydrolase, E. C. 3.5.1.1) has been proven to be competent in treating Acute Lymphoblastic Leukaemia (ALL), which is widely observed in paediatric and adult groups. Currently, clinical L-Asparaginase formulations are derived from bacterial sources such as Escherichia coli and Erwinia chrysanthemi. These formulations when administered to ALL patients lead to several immunological and hypersensitive reactions. Hence, additional purification steps are required to remove toxicity induced by the amalgamation of other enzymes like glutaminase and urease. Production of L-Asparaginase that is free of glutaminase and urease is a major area of research. In this paper, we report the screening and isolation of fungal species collected from the soil and mosses in the Schirmacher Hills, Dronning Maud Land, Antarctica, that produce L-Asparaginase free of glutaminase and urease. A total of 55 isolates were obtained from 33 environmental samples that were tested by conventional plate techniques using Phenol red and Bromothymol blue as indicators. Among the isolated fungi, 30 isolates showed L-Asparaginase free of glutaminase and urease. The L-Asparaginase producing strain Trichosporon asahii IBBLA1, which showed the highest zone index, was then optimized with a Taguchi design. Optimum enzyme activity of 20.57 U mL(-1) was obtained at a temperature of 30 degrees C and pH of 7.0 after 60 hours. Our work suggests that isolation of fungi from extreme environments such as Antarctica may lead to an important advancement in therapeutic applications with fewer side effects.
类型	Article
语种	英语
国家	India
开放获取类型	Green Published, Green Accepted, gold
收录类别	SCI-E
WOS记录号	WOS:000457753000020
WOS关键词	ACUTE LYMPHOBLASTIC-LEUKEMIA ; SCHIRMACHER OASIS ; OPTIMIZATION ; FUNGI ; IDENTIFICATION ; FERMENTATION ; PURIFICATION ; BACTERIUM
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218751
作者单位	1.Indian Inst Technol Hyderabad, Dept Chem Engn, IBBL, Sangareddy 502285, Telangana, India; 2.Indian Inst Technol Hyderabad, Dept Civil Engn, ECG, Sangareddy 502285, Telangana, India; 3.NCPOR, Vasco Da Gama 403804, Goa, India
推荐引用方式 GB/T 7714	Ashok, Anup,Doriya, Kruthi,Rao, Jyothi Vithal,et al. Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss[J],2019,9.
APA	Ashok, Anup,Doriya, Kruthi,Rao, Jyothi Vithal,Qureshi, Asif,Tiwari, Anoop Kumar,&Kumar, Devarai Santhosh.(2019).Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss.SCIENTIFIC REPORTS,9.
MLA	Ashok, Anup,et al."Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and Moss".SCIENTIFIC REPORTS 9(2019).