干旱区生态环境知识资源中心(Arid): De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms

Arid

DOI	10.1038/s41598-018-35891-6
	De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms
	Sarwar, Muhammad Bilal 1,2; Ahmad, Zarnab 1; Rashid, Bushra 1; Hassan, Sameera 1; Gregersen, Per L.2; Leyva, Maria De la O.2; Nagy, Istvan 2; Asp, Torben 2; Husnain, Tayyab 1
通讯作者	Rashid, Bushra
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2019
卷号	9
英文摘要	Agave, monocotyledonous succulent plants, is endemic to arid regions of North America, exhibiting exceptional tolerance to their xeric environments. They employ various strategies to overcome environmental constraints, such as crassulacean acid metabolism, wax depositions, and protective leaf morphology. Genomic resources of Agave species have received little attention irrespective of their cultural, economic and ecological importance, which so far prevented the understanding of the molecular bases underlying their adaptations to the arid environment. In this study, we aimed to elucidate molecular mechanism(s) using transcriptome sequencing of A. sisalana. A de novo approach was applied to assemble paired-end reads. The expression study unveiled 3,095 differentially expressed unigenes between well-irrigated and drought-stressed leaf samples. Gene ontology and KEGG analysis specified a significant number of abiotic stress responsive genes and pathways involved in processes like hormonal responses, antioxidant activity, response to stress stimuli, wax biosynthesis, and ROS metabolism. We also identified transcripts belonging to several families harboring important drought-responsive genes. Our study provides the first insight into the genomic structure of A. sisalana underlying adaptations to drought stress, thus providing diverse genetic resources for drought tolerance breeding research.
类型	Article
语种	英语
国家	Pakistan ; Denmark
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000456392400077
WOS关键词	READ ALIGNMENT ; PLANT ; GENE ; PHOTOSYNTHESIS ; MODULATION ; NETWORKS ; CUTICLE ; ROLES ; CAM
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218744
作者单位	1.Univ Punjab, Ctr Excellence Mol Biol, Plant Genom Lab, 87 West Canal Bank Rd, Lahore 53700, Pakistan; 2.Aarhus Univ, Dept Mol Biol & Genet, Forsogsvej 1, Slagelse, Denmark
推荐引用方式 GB/T 7714	Sarwar, Muhammad Bilal,Ahmad, Zarnab,Rashid, Bushra,et al. De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms[J],2019,9.
APA	Sarwar, Muhammad Bilal.,Ahmad, Zarnab.,Rashid, Bushra.,Hassan, Sameera.,Gregersen, Per L..,...&Husnain, Tayyab.(2019).De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms.SCIENTIFIC REPORTS,9.
MLA	Sarwar, Muhammad Bilal,et al."De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms".SCIENTIFIC REPORTS 9(2019).