干旱区生态环境知识资源中心(Arid): The ecdysone receptor complex is essential for the reproductive success in the female desert locust,Schistocerca gregaria

Arid

DOI	10.1038/s41598-018-36763-9
	The ecdysone receptor complex is essential for the reproductive success in the female desert locust,Schistocerca gregaria
	Lenaerts, Cynthia; Marchal, Elisabeth; Peeters, Paulien; Broeck, Jozef Vanden
通讯作者	Lenaerts, Cynthia
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2019
卷号	9
英文摘要	Ecdysteroid hormones influence the development and reproduction of arthropods by binding a heterodimeric complex of nuclear receptors, the ecdysone receptor (EcR) and the retinoid-X-receptor/ultraspiracle (RXR/USP). Here, we report on the in vivo role(s) of the ecdysone receptor complex, SchgrEcR/SchgrRXR, in the female reproductive physiology of a major phytophagous pest insect, i.e. the desert locust, Schistocerca gregaria. Tissue and temporal distribution profiles were analysed during the first gonadotrophic cycle of adult female locusts. RNA interference was used as a reverse genetics tool to investigate the in vivo role of the ecdysone receptor complex in ovarian maturation, oogenesis, fertility and fecundity. We discovered that silencing the ecdysone receptor complex in S. gregaria resulted in impaired ovulation and oviposition, indicative for a crucial role of this complex in chorion formation. We also found evidence for a feedback of SchgrEcR/SchgrRXR on juvenile hormone biosynthesis by the corpora allata. Furthermore, we observed a tissue-dependent effect of the SchgrEcR/SchgrRXR knockdown on the transcript levels of the insulin receptor and neuroparsin 3 and 4. The insulin receptor transcript levels were upregulated in the brain, but not the fat body and gonads. Neuroparsins 3 and 4 transcript levels were down regulated in the brain and fat body, but not in the gonads.
类型	Article
语种	英语
国家	Belgium
开放获取类型	Green Published, gold, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000455346100015
WOS关键词	JUVENILE-HORMONE ; OVARIAN DEVELOPMENT ; DROSOPHILA ; LOCUST ; EXPRESSION ; PROTEINS ; GENES ; ECR ; ECDYSTEROIDS ; MOSQUITO
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218743
作者单位	Katholieke Univ Leuven, Mol & Dev Physiol & Signal Transduct Res Grp, Naamsestr 59,POB 02465, B-3000 Leuven, Belgium
推荐引用方式 GB/T 7714	Lenaerts, Cynthia,Marchal, Elisabeth,Peeters, Paulien,et al. The ecdysone receptor complex is essential for the reproductive success in the female desert locust,Schistocerca gregaria[J],2019,9.
APA	Lenaerts, Cynthia,Marchal, Elisabeth,Peeters, Paulien,&Broeck, Jozef Vanden.(2019).The ecdysone receptor complex is essential for the reproductive success in the female desert locust,Schistocerca gregaria.SCIENTIFIC REPORTS,9.
MLA	Lenaerts, Cynthia,et al."The ecdysone receptor complex is essential for the reproductive success in the female desert locust,Schistocerca gregaria".SCIENTIFIC REPORTS 9(2019).