干旱区生态环境知识资源中心(Arid): Effect of treated wastewater irrigation in East Central region of Tunisia (Monastir governorate) on the biochemical and transcriptomic response of earthworms Eisenia andrei

Arid

DOI	10.1016/j.scitotenv.2018.07.449
	Effect of treated wastewater irrigation in East Central region of Tunisia (Monastir governorate) on the biochemical and transcriptomic response of earthworms Eisenia andrei
	Mkhinini, Marouane 1; Boughattas, Iteb 1; Alphonse, Vanessa 2; Livet, Alexandre 2; Bousserrhine, Noureddine 2; Banni, Mohammed 1
通讯作者	Boughattas, Iteb
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2019
卷号	647 页码:1245-1255
英文摘要	Treated wastewater (TWW) reuse for irrigation has become an excellent way to palliate water scarcity in Mediterranean arid regions. However, the toxicological effects of these effluents on the soil's organisms, especially earthworms, have not been well studied as yet. In this paper, earthworms Eisenia andrei were exposed for 7 days and 14 days to five agricultural soils irrigated with TWW for different periods: 1 year, 8 years, and 20 years. In addition, they were also exposed to soil from one reference site sampled from the Ouardenin perimeter in the Monastir Governorate in Tunisia. The effect on earthworms was assessed at the biochemical level by evaluating for catalase (CAT), glutathione-S-transferase (GST), malondialdehyde accumulation (MDA) and acetylcholinesterase inhibition (AChE). On the other hand, genotoxicity and transcriptomic responses were evaluated using micronuclei test (MNT) and gene expression level of CAT and GST. Moreover, metals uptake by earthworms was analyzed. Results showed that CAT and GST activity in the earthworm increased significantly when they were exposed to soils irrigated with TWW for 1, 8 and 20 years. Furthermore, MDA concentration also increased significantly with the increase in exposure period. However, AChE activity decreased and MNi frequency increased in earthworms after 7 and 14 days of exposure to soils irrigated with TWW for more than a year. The gene expression level of CAT and GST showed a significant variability, thus data are discussed in relation to the studied biomarkers (CAT and GST). These data provide new insights into the effect of toxicity of TWW on the soil's macro fauna, which is strongly affected by the trace elements and other organic compounds accumulated in soils after 20 years of TWW irrigation. (C) 2018 Elsevier B.V. All rights reserved.
英文关键词	Treated wastewater Eisenia andrei Oxidative stress Gene expression level Genotoxicity
类型	Article
语种	英语
国家	Tunisia ; France
收录类别	SCI-E
WOS记录号	WOS:000447090400126
WOS关键词	OLIVE MILL WASTE ; GLUTATHIONE S-TRANSFERASES ; HEAVY-METAL CONTAMINATION ; REAL-TIME PCR ; SOIL PROPERTIES ; OXIDATIVE STRESS ; DNA-DAMAGE ; APORRECTODEA-CALIGINOSA ; CHEMICAL-PROPERTIES ; RISK-ASSESSMENT
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218586
作者单位	1.Higher Inst Agron Chott Meriem, Lab Biochem & Environm Toxicol, Chott Meriem 4042, Tunisia; 2.Univ Paris Est Creteil, Lab Water Environm & Urban syst, F-94010 Creteil, France
推荐引用方式 GB/T 7714	Mkhinini, Marouane,Boughattas, Iteb,Alphonse, Vanessa,et al. Effect of treated wastewater irrigation in East Central region of Tunisia (Monastir governorate) on the biochemical and transcriptomic response of earthworms Eisenia andrei[J],2019,647:1245-1255.
APA	Mkhinini, Marouane,Boughattas, Iteb,Alphonse, Vanessa,Livet, Alexandre,Bousserrhine, Noureddine,&Banni, Mohammed.(2019).Effect of treated wastewater irrigation in East Central region of Tunisia (Monastir governorate) on the biochemical and transcriptomic response of earthworms Eisenia andrei.SCIENCE OF THE TOTAL ENVIRONMENT,647,1245-1255.
MLA	Mkhinini, Marouane,et al."Effect of treated wastewater irrigation in East Central region of Tunisia (Monastir governorate) on the biochemical and transcriptomic response of earthworms Eisenia andrei".SCIENCE OF THE TOTAL ENVIRONMENT 647(2019):1245-1255.