干旱区生态环境知识资源中心(Arid): Effects of Simulated Nitrogen Deposition and Precipitation Manipulation on Soil Microorganisms in the Desert Steppe of Northern China

Arid

DOI	10.1590/18069657rbcs20180031
	Effects of Simulated Nitrogen Deposition and Precipitation Manipulation on Soil Microorganisms in the Desert Steppe of Northern China
	Jia Meiqing 1; Huang Jing 2; Yang Yinghua 2; Han Guodong3 ; Zhang Guogang 2
通讯作者	Zhang Guogang
来源期刊	REVISTA BRASILEIRA DE CIENCIA DO SOLO
ISSN	0100-0683
出版年	2019
卷号	43
英文摘要	Soil microorganisms are influenced by climate change. However, the effect of climate change on soil cultivable bacteria are unclear. In this study, the composition and diversity of the soil cultivable bacterial community were explored by a dilution-plate method, PCR, and 16S rRNA sequencing in a desert steppe of northern China after repeated NH4NO3 amendments and precipitation manipulation for seven years. The experimental treatments were as follows: control (CK), N addition (+N), N addition plus water addition (+N+W), and N addition plus water reduction (+N-W). Among the treatment groups, 11 genera and 17 bacterial species were isolated. Nitrogen addition and precipitation manipulation significantly increased the number of cultivable bacteria in the 0.00-0.30 m layer compared to CK. Compared to +N treatment, the +N+W and +N-W treatments had no significant impact on the number of cultivable bacteria. Compared to the CK community, bacterial communities exposed to the other three treatments did not show shifts in the relative abundance of dominant genera and other cultivable bacteria, except for Pontibacter and Staphylococcus. The treatments +N+W and +N-W significantly modified the relative abundance of Pontibacter and Staphylococcus compared to the +N treatment. Available potassium and phosphorus, and moisture content contributed to the change in the composition of the cultivable bacterial community (p>0.05). Nitrogen addition and precipitation manipulation significantly decreased species richness in the 0.00-0.02 m layer, but they did not affect evenness and the Shannon-Wiener Index in the 0.00-0.30 m layer. This study provides insights into how the composition and diversity of the bacterial community is affected by climate change scenarios.
英文关键词	soil microorganisms climate change 16S rRNA
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Submitted, Green Published
收录类别	SCI-E
WOS记录号	WOS:000453871500001
WOS关键词	MICROBIAL COMMUNITY COMPOSITION ; WATER AVAILABILITY ; BACTERIAL COMMUNITIES ; FUNCTIONAL DIVERSITY ; PLANT COMMUNITY ; RESPONSES ; GRASSLAND ; BIOMASS ; INPUTS ; RESPIRATION
WOS类目	Soil Science
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218476
作者单位	1.Tianjin Normal Univ, Key Lab Water Resource & Environm, Tianjin, Peoples R China; 2.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin, Peoples R China; 3.Inner Mongolia Agr Univ, Coll Ecol & Environm Sci, Key Lab Grassland Resources, Minist Educ, Hottot, Peoples R China
推荐引用方式 GB/T 7714	Jia Meiqing,Huang Jing,Yang Yinghua,et al. Effects of Simulated Nitrogen Deposition and Precipitation Manipulation on Soil Microorganisms in the Desert Steppe of Northern China[J],2019,43.
APA	Jia Meiqing,Huang Jing,Yang Yinghua,Han Guodong,&Zhang Guogang.(2019).Effects of Simulated Nitrogen Deposition and Precipitation Manipulation on Soil Microorganisms in the Desert Steppe of Northern China.REVISTA BRASILEIRA DE CIENCIA DO SOLO,43.
MLA	Jia Meiqing,et al."Effects of Simulated Nitrogen Deposition and Precipitation Manipulation on Soil Microorganisms in the Desert Steppe of Northern China".REVISTA BRASILEIRA DE CIENCIA DO SOLO 43(2019).