干旱区生态环境知识资源中心(Arid): Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria

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DOI	10.1111/rec.12920
	Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria
	Giraldo-Silva, Ana 1,2; Nelson, Corey 1,2; Barger, Nichole N.3; Garcia-Pichel, Ferran 1,2
通讯作者	Garcia-Pichel, Ferran
来源期刊	RESTORATION ECOLOGY
ISSN	1061-2971
EISSN	1526-100X
出版年	2019
卷号	27 期号:4 页码:793-803
英文摘要	Biological soil crusts (biocrust) are microbial communities that develop at the soil surface of drylands and play an important role in erosion control and fertility. Soil surface disturbance from a broad range of natural and human processes (e.g. fire, livestock grazing, off-road traffic) cause significant losses in biocrust cover and associated ecosystems services. Hence, biocrust restoration is emerging as an important intervention strategy to rehabilitate degraded dryland soils. In a multistep process, we designed protocols for the establishment of microbial nurseries to produce photosynthetic cyanobacterial inoculum for biocrust seeding at scale. We first report on the strategy for isolation, directly from the target site, of a large culture collection of cyanobacteria that included multiple representatives of the five most common biocrust taxa. After genetic pedigreeing of these isolates, we could select those that best matched field populations genetically for scale-up cultivation. We then developed protocols for effective cyanobacterial biomass production to obtain sufficient inoculum. This was followed by conditioning treatments (hardening off) to preacclimate this inoculum to the stressful conditions expected in the field. Finally, we show that the inoculum obtained was fit to thrive in its original soil under natural outdoor conditions if sufficient water was available. We repeated this process successfully for four sites, two in the hot Chihuanuan desert and two in the cooler Great Basin Desert, and on two textural types of soils in each. The cyanobacterial biocrust nursery approach represents a versatile, viable, and safe tool for the rehabilitation of dryland soils.
英文关键词	biocrusts biological soil crusts cultivation cyanobacteria degraded drylands soils erosion control soil microbiome soil restoration
类型	Article
语种	英语
国家	USA
收录类别	SCI-E
WOS记录号	WOS:000474808600013
WOS关键词	BIOLOGICAL SOIL CRUSTS ; COLORADO PLATEAU ; ALGAL CRUSTS ; DESERT ; DIVERSITY ; DISTURBANCE ; COMMUNITY ; RECOVERY ; CULTURE ; ESTABLISHMENT
WOS类目	Ecology
WOS研究方向	Environmental Sciences & Ecology
来源机构	Arizona State University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218456
作者单位	1.Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA; 2.Arizona State Univ, Biodesign Inst, Ctr Fundamental & Appl Microbi, Tempe, AZ 85287 USA; 3.Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA
推荐引用方式 GB/T 7714	Giraldo-Silva, Ana,Nelson, Corey,Barger, Nichole N.,et al. Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria[J]. Arizona State University,2019,27(4):793-803.
APA	Giraldo-Silva, Ana,Nelson, Corey,Barger, Nichole N.,&Garcia-Pichel, Ferran.(2019).Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria.RESTORATION ECOLOGY,27(4),793-803.
MLA	Giraldo-Silva, Ana,et al."Nursing biocrusts: isolation, cultivation, and fitness test of indigenous cyanobacteria".RESTORATION ECOLOGY 27.4(2019):793-803.