干旱区生态环境知识资源中心(Arid): Can exposure to neem oil affect the spermatogenesis of predator Ceraeochrysa claveri?

Arid

DOI	10.1007/s00709-018-1329-7
	Can exposure to neem oil affect the spermatogenesis of predator Ceraeochrysa claveri?
	Gimenes Garcia, Ana Silvia 1; Scudeler, Elton Luiz 1; Felipe Pinheiro, Patricia Fernanda 2; dos Santos, Daniela Carvalho 1,3
通讯作者	dos Santos, Daniela Carvalho
来源期刊	PROTOPLASMA
ISSN	0033-183X
EISSN	1615-6102
出版年	2019
卷号	256 期号:3 页码:693-701
英文摘要	Novel biological control methods and integrated pest management strategies are basic requirements for the development of sustainable agriculture. As a result, there is a growing demand for research on the use of plant extracts and natural enemies such as the green lacewing, Ceraeochrysa claveri, as natural pest control methods. Studies have shown that although natural compounds such as neem oil (Azadirachta indica) are effective as pest control strategies, they also cause sublethal effects on nontarget insects, such as C. claveri. The aim of this study was to examine the effects of neem oil on C. claveri testes. C. claveri larvae were fed Diatraea saccharalis eggs, which were pretreated with 0.5%, 1%, and 2% neem oil. Testes were collected from larvae, pupae, and adults and analyzed using light and electron (transmission and scanning) microscopy. Changes in cellular stress and possible cell death were also determined by TUNEL assay and the marker HSP-70. The results showed that neem oil affects the organization and distribution of cysts in the testes and the normal sequence of cyst development, causing a delay in spermatogenesis in the testes of treated insects. Tests for cellular stress and DNA fragmentation indicated there was no cellular alteration in the treated groups. Although neem oil does not induce cell death or changes in HSP-70 expression, this biopesticide negatively impacts the process of spermatogenesis and could decrease the perpetuation of this species in the agroecosystem, indicating that the use of neem oil in association with green lacewings as a biological control should be carefully evaluated.
英文关键词	Biopesticide Green lacewing Morphology Testes Ultrastructure
类型	Article
语种	英语
国家	Brazil
收录类别	SCI-E
WOS记录号	WOS:000465637300011
WOS关键词	MALE REPRODUCTIVE-SYSTEM ; INDICA A. JUSS ; SCHISTOCERCA-GREGARIA ; CELL-DEATH ; SPERM ULTRASTRUCTURE ; DESERT LOCUST ; BINDING-SITES ; AZADIRACHTIN ; SPERMIOGENESIS ; MIDGUT
WOS类目	Plant Sciences ; Cell Biology
WOS研究方向	Plant Sciences ; Cell Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218195
作者单位	1.UNESP Sao Paulo State Univ, Lab Insects, Dept Morphol, Inst Biosci Botucatu, BR-18618689 Botucatu, SP, Brazil; 2.UNESP Sao Paulo State Univ, Dept Anat, Inst Biosci Botucatu, Botucatu, SP, Brazil; 3.UNESP Sao Paulo State Univ, Electron Microscopy Ctr, Inst Biosci Botucatu, Botucatu, SP, Brazil
推荐引用方式 GB/T 7714	Gimenes Garcia, Ana Silvia,Scudeler, Elton Luiz,Felipe Pinheiro, Patricia Fernanda,et al. Can exposure to neem oil affect the spermatogenesis of predator Ceraeochrysa claveri?[J],2019,256(3):693-701.
APA	Gimenes Garcia, Ana Silvia,Scudeler, Elton Luiz,Felipe Pinheiro, Patricia Fernanda,&dos Santos, Daniela Carvalho.(2019).Can exposure to neem oil affect the spermatogenesis of predator Ceraeochrysa claveri?.PROTOPLASMA,256(3),693-701.
MLA	Gimenes Garcia, Ana Silvia,et al."Can exposure to neem oil affect the spermatogenesis of predator Ceraeochrysa claveri?".PROTOPLASMA 256.3(2019):693-701.