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DOI	10.1016/j.pep.2018.11.004
	Using response surface methodology optimize culture conditions for human lactoferrin production in desert Chlorella
	Wang, WenLun 1,2; Wu, Xiaomin 1; Tan, Jun 2; Zhu, Lingyun 1; Mou, Yun 2; Zhang, Dongyi 1; Gao, Jianfeng 2
通讯作者	Zhang, Dongyi ; Gao, Jianfeng
来源期刊	PROTEIN EXPRESSION AND PURIFICATION
ISSN	1046-5928
EISSN	1096-0279
出版年	2019
卷号	155 页码:130-135
英文摘要	To optimize the expression conditions for human lactoferrin production, we have constructed the transgenic chlorella with human lactoferrin named as GTD8A1-HLF, the original chlorella was separated from Gurbantunggut Desert in Xinjiang China. To further improve the production of human lactoferrin, a sequential methodology was used to optimize human lactoferrin production by GTD8A1-HLF. First, a screening trial using a Plackett-Burman design was done, and variables with statistically significant effects on human lactoferrin bio-production were identified. These were further optimized by central composite design experiments and response surface methodology. Finally, we found that the maximum human lactoferrin production (52.70 mg/L) was achieved under the following optimized conditions: Initial pH 5.0, NaNO3 concentration of 0.600 mol/L, FeSO4 concentration of 0.006 mol/L, and a CuSO4 concentration of 0.002 mol/L, with the other medium components constituting the basal culture medium BBM. The yield of HLF protein under optimized culture conditions was approximately 4-fold higher than that obtained by using the basal culture medium BBM. The findings are significant for the potential industrial use of GTD8A1-HLF.
英文关键词	Human Lactoferrin Plackett-Burman design Central composite design experiments Response surface methodology Desert Chlorella
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000456905400019
WOS关键词	BOVINE LACTOFERRIN ; PROTEIN-PRODUCTION ; TRANSGENIC RICE ; EXPRESSION ; YEAST ; ALGA ; ASPERGILLUS ; SYSTEM
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218194
作者单位	1.Natl Univ Def Technol, Coll Liberal Arts & Sci, Dept Biol & Chem, Changsha 410073, Hunan, Peoples R China; 2.Shihezi Univ, Coll Life Sci & Biotechnol, Shihezi 832000, Peoples R China
推荐引用方式 GB/T 7714	Wang, WenLun,Wu, Xiaomin,Tan, Jun,et al. Using response surface methodology optimize culture conditions for human lactoferrin production in desert Chlorella[J],2019,155:130-135.
APA	Wang, WenLun.,Wu, Xiaomin.,Tan, Jun.,Zhu, Lingyun.,Mou, Yun.,...&Gao, Jianfeng.(2019).Using response surface methodology optimize culture conditions for human lactoferrin production in desert Chlorella.PROTEIN EXPRESSION AND PURIFICATION,155,130-135.
MLA	Wang, WenLun,et al."Using response surface methodology optimize culture conditions for human lactoferrin production in desert Chlorella".PROTEIN EXPRESSION AND PURIFICATION 155(2019):130-135.