干旱区生态环境知识资源中心(Arid): Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis

Arid

DOI	10.1371/journal.pone.0219300
	Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis
	Eduardo Marques-Galvez, Jose 1,2; Morte, Asuncion 1,2; Navarro-Rodenas, Alfonso 1,2; Garcia-Carmona, Francisco 3; Perez-Gilabert, Manuela 3
通讯作者	Perez-Gilabert, Manuela
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2019
卷号	14 期号:7
英文摘要	Terfezia claveryi Chatin is a mycorrhizal fungus that forms ectendomycorrhizal associations with plants of Helianthemum genus. Its appreciated edibility and drought resistance make this fungus a potential alternative crop in arid and semiarid areas of the Mediterranean region. In order to increase the knowledge about the biology of this fungus in terms of mycorrhiza formation and response to drought stress, a catalase from T. claveryi (TcCAT-1) has been purified to apparent homogeneity and biochemically characterized; in addition, the expression pattern of this enzyme during different stages of T. claveryi biological cycle and under drought stress conditions are reported. The results obtained, together with the phylogenetic analysis and homology modeling, indicate that TcCAT-1 is a homotetramer large subunit size monofunctional-heme catalase belonging to Clade 2. The highest expression of this enzyme occurs in mature mycorrhiza, revealing a possible role in mycorrhiza colonization, but it is not upregulated under drought stress. However, the H2O2 content of mycorrhizal plants submitted to drought stress is lower than in well watered treatments, suggesting that mycorrhization improves the plant's oxidative stress response, although not via TcCAT-1 upregulation.
类型	Article
语种	英语
国家	Spain
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000484947800069
WOS关键词	TERFEZIA-CLAVERYI CHATIN ; HISTOCHEMICAL-LOCALIZATION ; HYDROGEN-PEROXIDE ; PLANTS ; ACCUMULATION ; ANTIOXIDANT ; DIVERSITY ; RESPONSES ; STRESS ; GENE
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218120
作者单位	1.Thader Biotechnol SL, Campus Espinardo, Murcia, Spain; 2.Univ Murcia, Fac Biol, Dept Biol Vegetal Bot, Campus Espinardo, Murcia, Spain; 3.Univ Murcia, Fac Biol, Dept Bioquim & Biol Mol A, Campus Espinardo, Murcia, Spain
推荐引用方式 GB/T 7714	Eduardo Marques-Galvez, Jose,Morte, Asuncion,Navarro-Rodenas, Alfonso,et al. Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis[J],2019,14(7).
APA	Eduardo Marques-Galvez, Jose,Morte, Asuncion,Navarro-Rodenas, Alfonso,Garcia-Carmona, Francisco,&Perez-Gilabert, Manuela.(2019).Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis.PLOS ONE,14(7).
MLA	Eduardo Marques-Galvez, Jose,et al."Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis".PLOS ONE 14.7(2019).