Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.1371/journal.pone.0215165 |
| Comparative analysis of the root and leaf transcriptomes in Chelidonium majus L. | |
| Pourmazaheri, Helen1,2; Soorni, Aboozar3; Kohnerouz, Bahram Baghban1; Dehaghi, Nafiseh Khosravi2; Kalantar, Enayatollah4; Omidi, Mansoor5; Naghavi, Mohammad Reza5 | |
| 通讯作者 | Kohnerouz, Bahram Baghban ; Dehaghi, Nafiseh Khosravi ; Naghavi, Mohammad Reza |
| 来源期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 出版年 | 2019 |
| 卷号 | 14期号:4 |
| 英文摘要 | Chelidonium majus is a traditional medicinal plant, which commonly known as a rich resource for the major benzylisoquinoline alkaloids (BIAs), including morphine, sanguinarine, and berberine. To understand the biosynthesis of C. majus BIAs, we performed de novo transcriptome sequencing of its leaf and root tissues using Illumina technology. Following comprehensive evaluation of de novo transcriptome assemblies produced with five programs including Trinity, Bridger, BinPacker, IDBA-tran, and Velvet/Oases using a series of k-mer sizes (from 25 to 91), BinPacker was found to produce the best assembly using a kmer of 25. This study reports the results of differential gene expression (DGE), functional annotation, gene ontology (GO) analysis, classification of transcription factor (TF) s, and SSR and miRNA discovery. Our DGE analysis identified 6,028 transcripts that were up-regulated in the leaf, and 4,722 transcripts that were up-regulated in the root. Further investigations showed that most of the genes involved in the BIA biosynthetic pathway are significantly expressed in the root compared to the leaf. GO analysis showed that the predominant GO domain is cellular component, while TF analysis found bHLH to be the most highly represented TF family. Our study further identified 10 SSRs, out of a total of 39,841, that showed linkage to five unigenes encoding enzymes in the BIA pathway, and 10 conserved miRNAs that were previously not detected in this plant. The comprehensive transcriptome information presented herein provides a foundation for further explorations on study of the molecular mechanisms of BIA synthesis in C. majus. |
| 类型 | Article |
| 语种 | 英语 |
| 国家 | Iran |
| 开放获取类型 | Green Submitted, gold, Green Published |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000464503800023 |
| WOS关键词 | BENZYLISOQUINOLINE ALKALOID BIOSYNTHESIS ; 1ST COMMITTED STEP ; MOLECULAR-CLONING ; NORCOCLAURINE SYNTHASE ; N-METHYLTRANSFERASE ; CULTURED-CELLS ; GENE FAMILY ; IN-VITRO ; EXPRESSION ; PROTEIN |
| WOS类目 | Multidisciplinary Sciences |
| WOS研究方向 | Science & Technology - Other Topics |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/218098 |
| 作者单位 | 1.Univ Tabriz, Dept Plant Breeding & Biotechnol, Coll Agr, Tabriz, Iran; 2.Alborz Univ Med Sci, Dept Pharmacognosy, Fac Pharm, Karaj, Iran; 3.Isfahan Univ Technol, Dept Biotechnol, Coll Agr, Esfahan, Iran; 4.Alborz Univ Med Sci, Dept Microbiol & Immunol, Fac Med, Karaj, Iran; 5.Univ Tehran, Agron & Plant Breeding Dept, Agr & Nat Resources Coll, Karaj, Iran |
| 推荐引用方式 GB/T 7714 | Pourmazaheri, Helen,Soorni, Aboozar,Kohnerouz, Bahram Baghban,et al. Comparative analysis of the root and leaf transcriptomes in Chelidonium majus L.[J],2019,14(4). |
| APA | Pourmazaheri, Helen.,Soorni, Aboozar.,Kohnerouz, Bahram Baghban.,Dehaghi, Nafiseh Khosravi.,Kalantar, Enayatollah.,...&Naghavi, Mohammad Reza.(2019).Comparative analysis of the root and leaf transcriptomes in Chelidonium majus L..PLOS ONE,14(4). |
| MLA | Pourmazaheri, Helen,et al."Comparative analysis of the root and leaf transcriptomes in Chelidonium majus L.".PLOS ONE 14.4(2019). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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