干旱区生态环境知识资源中心(Arid): Upper respiratory tract disease and associated diagnostic tests of mycoplasmosis in Alabama populations of Gopher tortoises, Gopherus polyphemus

Arid

DOI	10.1371/journal.pone.0214845
	Upper respiratory tract disease and associated diagnostic tests of mycoplasmosis in Alabama populations of Gopher tortoises, Gopherus polyphemus
	Goessling, Jeffrey M.1,5; Guyer, Craig 1; Godwin, James C.2; Hermann, Sharon M.1; Sandmeier, Franzisca C.3; Smith, Lora L.4; Mendonca, Mary T.1
通讯作者	Goessling, Jeffrey M.
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2019
卷号	14 期号:4
英文摘要	Upper respiratory tract disease (URTD) in North American tortoises (Gopherus) has been the focus of numerous laboratory and field investigations, yet the prevalence and importance of this disease remains unclear across many tortoise populations. Furthermore, much research has been focused on understanding diagnostic biomarkers of two known agents of URTD, Mycoplasma agassizii and Mycoplasma testudineum, yet the reliability and importance of these diagnostic biomarkers across populations is unclear. Gopher Tortoises (Gopherus polyphemus) have experienced significant declines and are currently protected range wide. Geographically, Alabama represents an important connection for Gopher Tortoise populations between the core and periphery of this species' distribution. Herein, we systematically sampled 197 Gopher Tortoises for URTD across seven sites in south-central and south-eastern Alabama. Plasma samples were assayed for antibodies to M. agassizii and M. testudineum; nasal lavage samples were assayed for the presence of viable pathogens as well as pathogen DNA. Lastly, animals were scored for the presence of external symptoms and nasal scarring consistent with URTD. External symptoms of URTD were present in G. polyphemus in all sites sampled in Alabama. There was no relationship between active symptoms of URTD and Mycoplasma antibodies, however the presence of URTD nasal scarring was positively related to M. agassizii antibodies (P = 0.032). For a single site that was sampled in three sequential years, seroprevalence to M. agassizii significantly varied among years (P < 0.0001). Mycoplasma agassizii DNA was isolated from four of the seven sites using quantitative PCR, yet none of the samples were culture positive for either of the pathogens. An analysis of disease status and condition indicated that there was a significant, positive relationship between the severity of URTD symptoms and relative body mass (P < 0.05). This study highlights the need for continued monitoring of disease in wild populations. Specifically, focus must be placed on identifying other likely pathogens and relevant biomarkers that may be important drivers of URTD in North American tortoises. Special consideration should be given to environmental contexts that may render wild populations more susceptible to disease.
类型	Article
语种	英语
国家	USA
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000463487500015
WOS关键词	SEASONAL ACCLIMATION ; DESERT TORTOISES ; AGASSIZII ; TESTUDINEUM ; MORTALITY ; IMMUNITY ; RANGE
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
来源机构	Colorado State University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218095
作者单位	1.Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA; 2.Auburn Univ, Alabama Nat Heritage Program, Auburn, AL 36849 USA; 3.Colorado State Univ, Dept Biol, Pueblo, CO USA; 4.Joseph W Jones Ecol Res Ctr, Newton, GA USA; 5.Eckerd Coll, Nat Sci Coll, St Petersburg, FL 33733 USA
推荐引用方式 GB/T 7714	Goessling, Jeffrey M.,Guyer, Craig,Godwin, James C.,et al. Upper respiratory tract disease and associated diagnostic tests of mycoplasmosis in Alabama populations of Gopher tortoises, Gopherus polyphemus[J]. Colorado State University,2019,14(4).
APA	Goessling, Jeffrey M..,Guyer, Craig.,Godwin, James C..,Hermann, Sharon M..,Sandmeier, Franzisca C..,...&Mendonca, Mary T..(2019).Upper respiratory tract disease and associated diagnostic tests of mycoplasmosis in Alabama populations of Gopher tortoises, Gopherus polyphemus.PLOS ONE,14(4).
MLA	Goessling, Jeffrey M.,et al."Upper respiratory tract disease and associated diagnostic tests of mycoplasmosis in Alabama populations of Gopher tortoises, Gopherus polyphemus".PLOS ONE 14.4(2019).