干旱区生态环境知识资源中心(Arid): Microbial diversity and soil physiochemical characteristic of higher altitude

Arid

DOI	10.1371/journal.pone.0213844
	Microbial diversity and soil physiochemical characteristic of higher altitude
	Kumar, Saurabh 1; Suyal, Deep Chandra 1; Yadav, Amit 2; Shouche, Yogesh 2; Goel, Reeta 1
通讯作者	Kumar, Saurabh
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2019
卷号	14 期号:3
英文摘要	Altitude is the major factor affecting both biodiversity and soil physiochemical properties of soil ecosystems. In order to understand the effect of altitude on soil physiochemical properties and bacterial diversity across the Himalayan cold desert, high altitude Gangotri soil ecosystem was studied and compared with the moderate altitude Kandakhal soil. Soil physiochemical analysis showed that altitude was positively correlated with soil pH, organic matter and total nitrogen content. However soil mineral nutrients and soil phosphorus were negatively correlated to the altitude. RT-PCR based analysis revealed the decreased bacterial and diazotrophic abundance at high altitude. Metagenomic study showed that Proteobacteria, Acidobacteria and Actinobacteria were dominant bacteria phyla at high altitude soil while Bacteroidetes and Fermicutes were found dominant at low altitude. High ratio of Gram-negative to Gram positive bacteria at Gangotri suggests the selective proliferation of Gram negative bacteria at high altitude with decrease in Gram positive bacteria. Moreover, Alphaproteobacteria was found more abundant at high altitude while the opposite was true for Betaproteobacteria. Abundance of Cytophaga, Flavobacterium and Bacteroides (CFB) were also found comparatively high at high altitude. Presence of many taxonomically unclassified sequences in Gangotri soil indicates the presence of novel bacterial diversity at high altitude. Further, isolation of bacteria through indigenously designed diffusion chamber revealed the existence of bacteria which has been documented in unculturable study of WIH (Western Indian Himalaya) but never been cultivated from WIH. Nevertheless, diverse functional free-living psychrotrophic diazotrophs were isolated only from the high altitude Gangotri soil. Molecular characterization revealed them as Arthrobacter humicola, Brevibacillus invocatus, Pseudomonas mandelii and Pseudomonas helmanticensis. Thus, this study documented the bacterial and psychrophilic diazotrophic diversity at high altitude and is an effort for exploration of low temperature bacteria in agricultural productivity with the target for sustainable hill agriculture.
类型	Article
语种	英语
国家	India
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000461316400035
WOS关键词	GANGOTRI GLACIER ; ELEVATIONAL GRADIENT ; BACTERIAL DIVERSITY ; COMMUNITIES ; MICROORGANISMS ; DECOMPOSITION ; TEMPERATURE ; HIMALAYAS ; PATTERNS ; DESERT
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218090
作者单位	1.Govind Ballabh Pant Univ Agr & Technol, Coll Basic Sci & Humanities, Dept Microbiol, Pantnagar, Uttarakhand, India; 2.Natl Ctr Cell Sci, Natl Ctr Microbial Resource, Pune, Maharashtra, India
推荐引用方式 GB/T 7714	Kumar, Saurabh,Suyal, Deep Chandra,Yadav, Amit,et al. Microbial diversity and soil physiochemical characteristic of higher altitude[J],2019,14(3).
APA	Kumar, Saurabh,Suyal, Deep Chandra,Yadav, Amit,Shouche, Yogesh,&Goel, Reeta.(2019).Microbial diversity and soil physiochemical characteristic of higher altitude.PLOS ONE,14(3).
MLA	Kumar, Saurabh,et al."Microbial diversity and soil physiochemical characteristic of higher altitude".PLOS ONE 14.3(2019).