干旱区生态环境知识资源中心(Arid): Acanthocytosis and brain damage in area postrema and choroid plexus: Description of novel signs of Loxosceles apachea envenomation in rats

Arid

DOI	10.1371/journal.pone.0211689
	Acanthocytosis and brain damage in area postrema and choroid plexus: Description of novel signs of Loxosceles apachea envenomation in rats
	Fernando Plenge-Tellechea, Luis; Daniel Hernandez-Ramos, Angel; Manuel Munoz, Juan; Barraza-Garza, Guillermo; Rico-Escobar, Edna; Melendez-Martinez, David
通讯作者	Fernando Plenge-Tellechea, Luis
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2019
卷号	14 期号:2
英文摘要	Loxocelism is a neglected medical problem that depends on its severity, can cause a cutaneous or viscero-cutaneous syndrome. This syndrome is characterized by hemostatic effects and necrosis, and the severity of the loxoscelism depends on the amount of venom injected, the zone of inoculation, and the species. In the Chihuahuan desert, the most abundant species is L. apachea. Its venom and biological effects are understudied, including neurological effects. Thus, our aim is to explore the effect of this regional species of medical interest in the United States-Mexico border community, using rat blood and central nervous system (CNS), particularly, two brain structures involved in brain homeostasis, Area postrema (AP) and Choroid plexus (PC). L. apachea specimens were collected and venom was obtained. Different venom concentrations (0, 0.178 and 0.87 mu g/g) were inoculated into Sprague-Dawley rats (intraperitoneal injection). Subsequently, blood was extracted and stained with Wright staining; coronal sections of AP were obtained and stained with Hematoxylin- Eosin (HE) staining and laminin. immunolabelling, the same was done with CP sections. Blood, AP and CP were observed under the microscope and abnormalities in erythrocytes and fluctuation in leukocyte types were described and quantified in blood. Capillaries were also quantified in AP and damage was described in CP. L. apachea venom produced a segmented neutrophil increment (neutrophilia), lymphocyte diminishment (leukopenia) and erythrocytes presented membrane abnormalities (acanthocytosis). Extravasated erythrocytes were observed in HE stained sections from both, AP and CP, which suggest that near to this section a hemorrhage is present; through immunohistofluorescence, a diminishment of laminin. was observed in AP endothelial cells and in CP ependymal cells when these structures were exposed to L. apachea venom. In conclusion, L. apachea venom produced leukopenia, netrophilia and acanthocytosis in rat peripheral blood, and also generated hemorrhages on AP and CP through degradation of laminin gamma.
类型	Article
语种	英语
国家	Mexico
开放获取类型	Green Submitted, Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000458026000044
WOS关键词	SPIDER VENOMS ; BROWN ; IDENTIFICATION ; TOXINS ; RECEPTORS ; CLONING
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218084
作者单位	Univ Autonoma Ciudad Juarez, Inst Ciencias Biomed, Dept Ciencias Quim Biol, Lab Biol Mol & Bioquim, Ciudad Juarez, Chihuahua, Mexico
推荐引用方式 GB/T 7714	Fernando Plenge-Tellechea, Luis,Daniel Hernandez-Ramos, Angel,Manuel Munoz, Juan,et al. Acanthocytosis and brain damage in area postrema and choroid plexus: Description of novel signs of Loxosceles apachea envenomation in rats[J],2019,14(2).
APA	Fernando Plenge-Tellechea, Luis,Daniel Hernandez-Ramos, Angel,Manuel Munoz, Juan,Barraza-Garza, Guillermo,Rico-Escobar, Edna,&Melendez-Martinez, David.(2019).Acanthocytosis and brain damage in area postrema and choroid plexus: Description of novel signs of Loxosceles apachea envenomation in rats.PLOS ONE,14(2).
MLA	Fernando Plenge-Tellechea, Luis,et al."Acanthocytosis and brain damage in area postrema and choroid plexus: Description of novel signs of Loxosceles apachea envenomation in rats".PLOS ONE 14.2(2019).