干旱区生态环境知识资源中心(Arid): Quality and quantity of dromedary camel DNA sampled from whole-blood, saliva, and tail-hair

Arid

DOI	10.1371/journal.pone.0211743
	Quality and quantity of dromedary camel DNA sampled from whole-blood, saliva, and tail-hair
	Alhaddad, Hasan 1; Maraqa, Tasneem 1; Alabdulghafour, Suha 1; Alaskar, Huda 1; Alaqeely, Randa 1; Almathen, Faisal 2,3; Alhajeri, Bader H.1
通讯作者	Alhaddad, Hasan
来源期刊	PLOS ONE
ISSN	1932-6203
出版年	2019
卷号	14 期号:1
英文摘要	Camels are livestock with unique adaptations to hot-arid regions. To effectively study camel traits, a biobank of camel DNA specimens with associated biological information is needed. We examined whole-blood, saliva (buccal swabs), and tail-hair follicle samples to determine which is the best source for establishing a DNA biobank. We inspected five amounts of each of whole-blood, buccal swabs, and tail-hair follicles in nine camels, both qualitatively via gel electrophoresis and quantitatively using a NanoDrop spectrophotometer. We also tested the effects of long term-storage on the quality and quantity of DNA, and measured the rate of degradation, by analyzing three buccal swab samples and 30 tail-hair follicles over a period of nine months. Good quality DNA, in the form of visible large size DNA bands, was extracted from all three sources, for all five amounts. The five volumes of whole-blood samples (20-100 mu l) provided similar to 0.4-3.6 mu g, the five quantities of buccal swabs (1-5) produced similar to 0.1-12 mu g, while the five amounts of tail-hair follicles (10-50) resulted in similar to 0.7-25 mu g. No differences in the rate of degradation of buccal swab and tail-hair follicle DNA were detected, but there was clearly greater deterioration in the quality of DNA extracted from buccal swabs when compared to tail-hair follicles. We recommend using tail-hair samples for camel DNA biobanking, because it resulted in both an adequate quality and quantity of DNA, along with its ease of collection, transportation, and storage. Compared to its success in studies of other domesticated animals, we anticipate that using similar to 50 tail-hair follicles will provide sufficient DNA for sequencing or SNP genotyping.
类型	Article
语种	英语
国家	Kuwait ; Saudi Arabia
开放获取类型	Green Submitted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000457301600066
WOS关键词	GENOME-WIDE ASSOCIATION ; GULF
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218080
作者单位	1.Kuwait Univ, Dept Biol Sci, Safat, Kuwait; 2.King Faisal Univ, Coll Vet Med, Dept Vet Publ Hlth & Anim Husb, Al Hasa, Saudi Arabia; 3.King Faisal Univ, Camel Res Ctr, Al Hasa, Saudi Arabia
推荐引用方式 GB/T 7714	Alhaddad, Hasan,Maraqa, Tasneem,Alabdulghafour, Suha,et al. Quality and quantity of dromedary camel DNA sampled from whole-blood, saliva, and tail-hair[J],2019,14(1).
APA	Alhaddad, Hasan.,Maraqa, Tasneem.,Alabdulghafour, Suha.,Alaskar, Huda.,Alaqeely, Randa.,...&Alhajeri, Bader H..(2019).Quality and quantity of dromedary camel DNA sampled from whole-blood, saliva, and tail-hair.PLOS ONE,14(1).
MLA	Alhaddad, Hasan,et al."Quality and quantity of dromedary camel DNA sampled from whole-blood, saliva, and tail-hair".PLOS ONE 14.1(2019).