干旱区生态环境知识资源中心(Arid): Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album

Arid

DOI	10.1371/journal.pgen.1008209
	Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album
	Wai, Ching Man 1; Weise, Sean E.2; Ozersky, Philip 3; Mockler, Todd C.3; Michael, Todd P.4; VanBuren, Robert 1,5
通讯作者	Michael, Todd P. ; VanBuren, Robert
来源期刊	PLOS GENETICS
ISSN	1553-7404
出版年	2019
卷号	15 期号:6
英文摘要	Plants with facultative crassulacean acid metabolism (CAM) maximize performance through utilizing C3 or C4 photosynthesis under ideal conditions while temporally switching to CAM under water stress (drought). While genome-scale analyses of constitutive CAM plants suggest that time of day networks are shifted, or phased to the evening compared to C3, little is known for how the shift from C3 to CAM networks is modulated in drought induced CAM. Here we generate a draft genome for the drought-induced CAM-cycling species Sedum album. Through parallel sampling in well-watered (C3) and drought (CAM) conditions, we uncover a massive rewiring of time of day expression and a CAM and stress-specific network. The core circadian genes are expanded in S. album and under CAM induction, core clock genes either change phase or amplitude. While the core clock cis-elements are conserved in S. album, we uncover a set of novel CAM and stress specific cis-elements consistent with our finding of rewired co-expression networks. We identified shared elements between constitutive CAM and CAM-cycling species and expression patterns unique to CAM-cycling S. album. Together these results demonstrate that drought induced CAM-cycling photosynthesis evolved through the mobilization of a stress-specific, time of day network, and not solely the phasing of existing C3 networks. These results will inform efforts to engineer water use efficiency into crop plants for growth on marginal land. Author summary Crassulacean acid metabolism (CAM) photosynthesis represents an important adaptation to arid environments as CAM plants take up CO2 at night when evapotranspiration rates are lower. Genomes and large-scale datasets are available for several plants with constitutive CAM activity, but they provided little insight on how this trait evolved from C3. Here we sequenced the CAM-cycling plant Sedum album, which switches from C3 to CAM photosynthesis under drought conditions. We performed a global gene expression analysis sampling every two hours over 24 hours in C3 (well-watered) and CAM (drought) conditions. This comparative approach allowed us to identify components of the CAM pathway that were previously unidentified in constitutive CAM plants such as pineapple and orchid. Our results reveal a massive time of day specific rewiring of the transcriptional networks in Sedum where only 20% of cycling genes overlap between the C3 and CAM conditions. This time of day reprogramming results in broad network changes linking stress pathways and photosynthesis under CAM.
类型	Article
语种	英语
国家	USA
开放获取类型	Green Published, gold, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000475464900030
WOS关键词	CRASSULACEAN ACID METABOLISM ; COMMON ICE PLANT ; WATER-USE ; MESEMBRYANTHEMUM-CRYSTALLINUM ; ANTIOXIDATIVE PROTECTION ; GENOME ; STARCH ; EVOLUTION ; ECOPHYSIOLOGY ; TRANSPORTERS
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218071
作者单位	1.Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA; 2.Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA; 3.Donald Danforth Plant Sci Ctr, St Louis, MO USA; 4.J Craig Venter Inst, La Jolla, CA 92037 USA; 5.Michigan State Univ, Plant Resilience Inst, E Lansing, MI 48824 USA
推荐引用方式 GB/T 7714	Wai, Ching Man,Weise, Sean E.,Ozersky, Philip,et al. Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album[J],2019,15(6).
APA	Wai, Ching Man,Weise, Sean E.,Ozersky, Philip,Mockler, Todd C.,Michael, Todd P.,&VanBuren, Robert.(2019).Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album.PLOS GENETICS,15(6).
MLA	Wai, Ching Man,et al."Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album".PLOS GENETICS 15.6(2019).