干旱区生态环境知识资源中心(Arid): Understanding the Role of the WRKY Gene Family under Stress Conditions in Pigeonpea (Cajanus Cajan L.)

Arid

DOI	10.3390/plants8070214
	Understanding the Role of the WRKY Gene Family under Stress Conditions in Pigeonpea (Cajanus Cajan L.)
	Singh, Akshay 1,2; Singh, Pankaj Kumar 1; Sharma, Ajay Kumar 3; Singh, Nagendra Kumar 4; Sonah, Humira 1; Deshmukh, Rupesh 1; Sharma, Tilak Raj 1
通讯作者	Sharma, Tilak Raj
来源期刊	PLANTS-BASEL
EISSN	2223-7747
出版年	2019
卷号	8 期号:7
英文摘要	Pigeonpea (Cajanus cajan L.), a protein-rich legume, is a major food component of the daily diet for residents in semi-arid tropical regions of the word. Pigeonpea is also known for its high level of tolerance against biotic and abiotic stresses. In this regard, understanding the genes involved in stress tolerance has great importance. In the present study, identification, and characterization of WRKY, a large transcription factor gene family involved in numerous biological processes like seed germination, metabolism, plant growth, biotic and abiotic stress responses was performed in pigeonpea. A total of 94 WRKY genes identified in the pigeonpea genome were extensively characterized for gene-structures, localizations, phylogenetic distribution, conserved motif organizations, and functional annotation. Phylogenetic analysis revealed three major groups (I, II, and III) of pigeonpea WRKY genes. Subsequently, expression profiling of 94 CcWRKY genes across different tissues like root, nodule, stem, petiole, petal, sepal, shoot apical meristem (SAM), mature pod, and mature seed retrieved from the available RNAseq data identified tissue-specific WRKY genes with preferential expression in the vegetative and reproductive stages. Gene co-expression networks identified four WRKY genes at the center of maximum interaction which may play a key role in the entire WRKY regulations. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) expression analysis of WRKY genes in root and leaf tissue samples from plants under drought and salinity stress identified differentially expressed WRKY genes. The study will be helpful to understand the evolution, regulation, and distribution of the WRKY gene family, and additional exploration for the development of stress tolerance cultivars in pigeonpea and other legumes crops.
英文关键词	transcription factors WRKY domain gene expressing profiling stress tolerance drought high salinity
类型	Article
语种	英语
国家	India
开放获取类型	Green Published, gold, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000481484800011
WOS关键词	TRANSCRIPTION FACTOR GENES ; MOLECULAR-CLONING ; DEFENSE RESPONSE ; ABSCISIC-ACID ; EXPRESSION ; ARABIDOPSIS ; RICE ; RESISTANCE ; SEQUENCES ; PROTEIN
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218066
作者单位	1.Natl Agrifood Biotechnol Inst, Mohali 140306, Punjab, India; 2.Dr APJ Abdul Kalam Tech Univ, Lucknow 226031, Uttar Pradesh, India; 3.Meerut Inst Engn & Technol, Meerut 250005, Uttar Pradesh, India; 4.Natl Inst Plant Biotechnol, New Delhi 110012, India
推荐引用方式 GB/T 7714	Singh, Akshay,Singh, Pankaj Kumar,Sharma, Ajay Kumar,et al. Understanding the Role of the WRKY Gene Family under Stress Conditions in Pigeonpea (Cajanus Cajan L.)[J],2019,8(7).
APA	Singh, Akshay.,Singh, Pankaj Kumar.,Sharma, Ajay Kumar.,Singh, Nagendra Kumar.,Sonah, Humira.,...&Sharma, Tilak Raj.(2019).Understanding the Role of the WRKY Gene Family under Stress Conditions in Pigeonpea (Cajanus Cajan L.).PLANTS-BASEL,8(7).
MLA	Singh, Akshay,et al."Understanding the Role of the WRKY Gene Family under Stress Conditions in Pigeonpea (Cajanus Cajan L.)".PLANTS-BASEL 8.7(2019).