干旱区生态环境知识资源中心(Arid): Transcriptome Sequencing of Chickpea (Cicer arietinum L.) Genotypes for Identification of Drought-Responsive Genes Under Drought Stress Condition

Arid

DOI	10.1007/s11105-019-01147-4
	Transcriptome Sequencing of Chickpea (Cicer arietinum L.) Genotypes for Identification of Drought-Responsive Genes Under Drought Stress Condition
	Kumar, Manoj 1,2; Chauhan, Abhishek Singh 1,3; Kumar, Manoj 1; Yusuf, Mohd Aslam 4; Sanyal, Indraneel 5; Chauhan, Puneet Singh 1
通讯作者	Chauhan, Puneet Singh
来源期刊	PLANT MOLECULAR BIOLOGY REPORTER
ISSN	0735-9640
EISSN	1572-9818
出版年	2019
卷号	37 期号:3 页码:186-203
英文摘要	Chickpea (Cicer arietinum L.) is a pulse crop valued for its high protein content, grown in semi-arid tropics and Mediterranean regions. Its yield remains affected by biotic and abiotic stresses with drought alone being responsible for up to 50% annual loss of yield. Transcriptome analysis of a sensitive and a tolerant cultivar of chickpea has been done earlier to unravel the molecular basis for drought and salinity stress responses. In the present study, we performed transcriptome analysis of two drought-tolerant genotypes, BG-362 and P-256, under polyethylene glycol-simulated drought stress to decipher the genes and pathways that are commonly regulated in these genotypes. RNA-Seq using Illumina platform generated 152 million high-quality reads. Reference-guided assembly of genome yielded a total of 37,943 transcripts representing 22,701 genes. Among the 1624 genes that were observed to be differentially expressed under drought, 97 genes were common in both the genotypes. These included the upregulated genes, such as probable mannitol dehydrogenase, serine hydroxymethyltransferase 4-like, 17.5kDa class I heat shock protein-like, cytochrome P450 81E8-like, and galactinol-sucrose galactosyltransferase-like, and downregulated genes, such as probable xyloglucan endotransglucosylase/hydrolase protein 23, abscisic acid 8-hydroxylase 1-like, Calmodulin-like protein 11, and proline dehydrogenase 2 mitochondrial-like genes. A major finding was the involvement of transcription factors, including AP2-EREBP, bHLH, bZIP, C3H, MYB, NAC, WRKY, and MADS. The present study is the first comparative analysis of RNA-Seq data for two drought-tolerant chickpea genotypes. These findings would help in improving drought tolerance across chickpea genotypes.
英文关键词	Chickpea Drought Root Transcriptome sequencing
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000472102800005
WOS关键词	HIGH-SALINITY STRESSES ; ABIOTIC STRESS ; EXPRESSION PROFILES ; INDUCIBLE GENES ; SALT STRESS ; TOLERANCE ; DEHYDRATION ; ARABIDOPSIS ; COLD ; NETWORKS
WOS类目	Biochemical Research Methods ; Plant Sciences
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218044
作者单位	1.CSIR, NBRI, Div Plant Microbe Interact, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, India; 2.Integral Univ, Dept Biosci, Lucknow 226026, Uttar Pradesh, India; 3.Acad Sci & Innovat Res, New Delhi, India; 4.Integral Univ, Dept Bioengn, Lucknow 226026, Uttar Pradesh, India; 5.CSIR, Natl Bot Res Inst, Genet & Plant Mol Div, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, India
推荐引用方式 GB/T 7714	Kumar, Manoj,Chauhan, Abhishek Singh,Kumar, Manoj,et al. Transcriptome Sequencing of Chickpea (Cicer arietinum L.) Genotypes for Identification of Drought-Responsive Genes Under Drought Stress Condition[J],2019,37(3):186-203.
APA	Kumar, Manoj,Chauhan, Abhishek Singh,Kumar, Manoj,Yusuf, Mohd Aslam,Sanyal, Indraneel,&Chauhan, Puneet Singh.(2019).Transcriptome Sequencing of Chickpea (Cicer arietinum L.) Genotypes for Identification of Drought-Responsive Genes Under Drought Stress Condition.PLANT MOLECULAR BIOLOGY REPORTER,37(3),186-203.
MLA	Kumar, Manoj,et al."Transcriptome Sequencing of Chickpea (Cicer arietinum L.) Genotypes for Identification of Drought-Responsive Genes Under Drought Stress Condition".PLANT MOLECULAR BIOLOGY REPORTER 37.3(2019):186-203.