干旱区生态环境知识资源中心(Arid): Introgression lines of Solanum sitiens, a wild nightshade of the Atacama Desert, in the genome of cultivated tomato

Arid

DOI	10.1111/tpj.14460
	Introgression lines of Solanum sitiens, a wild nightshade of the Atacama Desert, in the genome of cultivated tomato
	Chetelat, Roger T.1; Qin, Xiaoqiong 1; Tan, Meilian 1,2; Burkart-Waco, Diana 1; Moritama, Yosuke 1,3; Huo, Xiuwen 1,4; Wills, Tim 1,5; Pertuze, Ricardo 1,6
通讯作者	Chetelat, Roger T.
来源期刊	PLANT JOURNAL
ISSN	0960-7412
EISSN	1365-313X
出版年	2019
卷号	100 期号:4 页码:836-850
英文摘要	The wild tomato relative Solanum sitiens is a xerophyte endemic to the Atacama Desert of Chile and a potential source of genes for tolerance to drought, salinity and low-temperature stresses. However, until recently, strong breeding barriers prevented its hybridization and introgression with cultivated tomato, Solanum lycopersicum L. We overcame these barriers using embryo rescue, bridging lines and allopolyploid hybrids, and synthesized a library of introgression lines (ILs) that captures the genome of S. sitiens in the background of cultivated tomato. The IL library consists of 56 overlapping introgressions that together represent about 93% of the S. sitiens genome: 65% in homozygous and 28% in heterozygous (segregating) ILs. The breakpoints of each segment and the gaps in genome coverage were mapped by single nucleotide polymorphism (SNP) genotyping using the SolCAP SNP array. Marker-assisted selection was used to backcross selected introgressions into tomato, to recover a uniform genetic background, to isolate recombinant sub-lines with shorter introgressions and to select homozygous genotypes. Each IL contains a single S. sitiens chromosome segment, defined by markers, in the genetic background of cv. NC 84173, a fresh market inbred line. Large differences were observed between the lines for both qualitative and quantitative morphological traits, suggesting that the ILs contain highly divergent allelic variation. Several loci contributing to unilateral incompatibility or hybrid necrosis were mapped with the lines. This IL population will facilitate studies of the S. sitiens genome and expands the range of genetic variation available for tomato breeding and research.
英文关键词	tomato Solanum sitiens wide hybridization unilateral incompatibility hybrid incompatibility homeologous recombination
类型	Article
语种	英语
国家	USA ; Peoples R China ; Japan ; Chile
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000486652600001
WOS关键词	LYCOPERSICON-ESCULENTUM ; HYBRID INCOMPATIBILITY ; SELF-INCOMPATIBILITY ; UNILATERAL INCOMPATIBILITY ; POLLEN REJECTION ; IDENTIFICATION ; BACKCROSS ; GENETICS ; DIFFERENTIATION ; RECOMBINATION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	University of California, Davis
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218042
作者单位	1.Univ Calif Davis, CM Rick Tomato Genet Resource Ctr, Dept Plant Sci, Davis, CA 95616 USA; 2.Chinese Acad Agr Sci, Oil Crops Res Inst, Xudong 2nd Rd, Wuhan 430062, Hubei, Peoples R China; 3.Sakata Seed Corp, Kimitsu Res Stn, 358 Uchikoshi, Sodegaura, Chiba, Japan; 4.Inner Mongolia Agr Univ, Coll Agron, 306 Zhaowuda Rd, Hohhot 010019, Inner Mongolia, Peoples R China; 5.Univ Orlando Resort, Hort Dept, 1000 Universal Studios Plaza, Orlando, FL 32821 USA; 6.Univ Chile, Fac Ciencias Agron, Dept Prod Agr, Casilla 1004, Santiago, Chile
推荐引用方式 GB/T 7714	Chetelat, Roger T.,Qin, Xiaoqiong,Tan, Meilian,et al. Introgression lines of Solanum sitiens, a wild nightshade of the Atacama Desert, in the genome of cultivated tomato[J]. University of California, Davis,2019,100(4):836-850.
APA	Chetelat, Roger T..,Qin, Xiaoqiong.,Tan, Meilian.,Burkart-Waco, Diana.,Moritama, Yosuke.,...&Pertuze, Ricardo.(2019).Introgression lines of Solanum sitiens, a wild nightshade of the Atacama Desert, in the genome of cultivated tomato.PLANT JOURNAL,100(4),836-850.
MLA	Chetelat, Roger T.,et al."Introgression lines of Solanum sitiens, a wild nightshade of the Atacama Desert, in the genome of cultivated tomato".PLANT JOURNAL 100.4(2019):836-850.