干旱区生态环境知识资源中心(Arid): Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum)

Arid

DOI	10.1094/PDIS-12-18-2164-RE
	Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum)
	Fribourg, Cesar E.1; Gibbs, Adrian J.2; Adams, Ian P.3; Boonham, Neil 4; Jones, Roger A. C.5,6
通讯作者	Jones, Roger A. C.
来源期刊	PLANT DISEASE
ISSN	0191-2917
EISSN	1943-7692
出版年	2019
卷号	103 期号:7 页码:1746-1756
英文摘要	In 1976, a virus with flexuous, filamentous virions typical of the family Potyviridae was isolated from symptomatic pepino (Solanum muricatum) plants growing in two valleys in Peru's coastal desert region. In 2014, a virus with similar-shaped virions was isolated from asymptomatic fruits obtained from pepino plants growing in six coastal valleys and a valley in Peru's Andean highlands. Both were identified subsequently as Wild potato mosaic virus (WPMV) by serology or high-throughput sequencing (HTS). The symptoms caused by two old and seven new isolates from pepino were examined in indicator plants. Infected solanaceous hosts varied considerably in their sensitivities to infection and individual isolates varied greatly in virulence. All seven new isolates caused quick death of infected Nicotiana benthamiana plants and more than half of them killed infected plants of Physalis floridana and S. chancayense. These three species were the most sensitive to infection. The most virulent isolate was found to be BA because it killed five of eight solanaceous host species whereas CA was the least severe because it only killed N. benthamiana. Using HTS, complete genomic sequences of six isolates were obtained, with one isolate (FE) showing evidence of recombination. The distances between individual WPMV isolates in phylogenetic trees and the geographical distances between their collection sites were found to be unrelated. The individual WPMV isolates displayed nucleotide sequence identities of 80.9-99.8%, whereas the most closely related virus, Potato virus V (PVV), was around 75% identical to WPMV. WPMV, PVV, and Peru tomato virus formed clusters of similar phylogenetic diversity, and were found to be distinct but related viruses within the overall Potato virus Y lineage. WPMV infection seems widespread and of likely economic significance to pepino producers in Peru's coastal valleys. Because it constitutes the fifth virus found infecting pepino and this crop is entirely vegetatively propagated, development of healthy pepino stock programs is advocated.
英文关键词	American lineage disease management economic significance etiology high-throughput sequencing host sensitivity to infection isolate virulence pathogen detection pathogen diversity pepino Peru phylogenetic analysis bush fruit symptomatology virions viruses and viroids widespread infection Wild potato mosaic virus
类型	Article
语种	英语
国家	Peru ; Australia ; England
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000471737000034
WOS关键词	HOST-PLANT REACTIONS ; MOTTLE-VIRUS ; RESISTANCE ; SEQUENCE ; TOMATO ; RECOMBINATION ; STRAIN ; POTYVIRUSES ; ALIGNMENT ; GENETICS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
来源机构	University of Western Australia
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/218028
作者单位	1.Univ Nacl Agr, Dept Fitopatol, Lima, Peru; 2.Australian Natl Univ, Emeritus Fac, Canberra, ACT, Australia; 3.Fera Sci Ltd, York, N Yorkshire, England; 4.Newcastle Univ, Inst Agrifood Res Innovat, Newcastle Upon Tyne, Tyne & Wear, England; 5.Univ Western Australia, Inst Agr, 35 Stirling Highway, Crawley, WA, Australia; 6.Dept Primary Ind & Reg Dev, 3 Baron Hay Court, S Perth, WA, Australia
推荐引用方式 GB/T 7714	Fribourg, Cesar E.,Gibbs, Adrian J.,Adams, Ian P.,et al. Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum)[J]. University of Western Australia,2019,103(7):1746-1756.
APA	Fribourg, Cesar E.,Gibbs, Adrian J.,Adams, Ian P.,Boonham, Neil,&Jones, Roger A. C..(2019).Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum).PLANT DISEASE,103(7),1746-1756.
MLA	Fribourg, Cesar E.,et al."Biological and Molecular Properties of Wild potato mosaic virus Isolates from Pepino (Solanum muricatum)".PLANT DISEASE 103.7(2019):1746-1756.