干旱区生态环境知识资源中心(Arid): Functional Analysis of Ion Transport Properties and Salt Tolerance Mechanisms of RtHKT1 from the Recretohalophyte Reaumuria trigyna

Arid

DOI	10.1093/pcp/pcy187
	Functional Analysis of Ion Transport Properties and Salt Tolerance Mechanisms of RtHKT1 from the Recretohalophyte Reaumuria trigyna
	Li, Ningning; Du, Chao; Ma, Binjie; Gao, Ziqi; Wu, Zhigang; Zheng, Linlin; Niu, Yiding; Wang, Yingchun
通讯作者	Niu, Yiding ; Wang, Yingchun
来源期刊	PLANT AND CELL PHYSIOLOGY
ISSN	0032-0781
EISSN	1471-9053
出版年	2019
卷号	60 期号:1 页码:85-106
英文摘要	Reaumuria trigyna is an endangered recretohalophyte and a small archaic feral shrub that is endemic to arid and semi-arid plateau regions of Inner Mongolia, China. Based on transcriptomic data, we isolated a high-affinity potassium transporter gene (RtHKT1) from R. trigyna, which encoded a plasma membrane-localized protein. RtHKT1 was rapidly up-regulated by high Na+ or low K+ and exhibited different tissue-specific expression patterns before and after stress treatment. Transgenic yeast showed tolerance to high Na+ or low K+, while transgenic Arabidopsis exhibited tolerance to high Na+ and sensitivity to high K+, or high Na+-low K+, confirming that Na+ tolerance in transgenic Arabidopsis depends on a sufficient external K+ concentration. Under external high Na+, high K+ and low K+ conditions, transgenic yeast accumulated more Na+-K+, Na+ and K+, while transgenic Arabidopsis accumulated less Na+-more K+, more Na+ and more Na+-K+, respectively, indicating that the ion transport properties of RtHKT1 depend on the external Na+-K+ environment. Salt stress induced up-regulation of some ion transporter genes (AtSOS1/AtHAK5/AtKUP5-6), as well as down-regulation of some genes (AtNHX1/AtAVP1/AtKUP9-12), revealing that multi-ion-transporter synergism maintains Na+/K+ homeostasis under salt stress in transgenic Arabidopsis. Overexpression of RtHKT1 enhanced K+ accumulation and prevented Na+ transport from roots to shoots, improved biomass accumulation and Chl content in salt-stressed transgenic Arabidopsis. The proline content and relative water content increased significantly, and some proline biosynthesis genes (AtP5CS1 and AtP5CS2) were also up-regulated in salt-stressed transgenic plants. These results suggest that RtHKT1 confers salt tolerance on transgenic Arabidopsis by maintaining Na+/K+ homeostasis and osmotic homeostasis.
英文关键词	High-affinity potassium transporter Ion homeostasis Ion transport properties Osmotic regulation Reaumuria trigyna Salt-tolerant transgenic Arabidopsis
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000459633500010
WOS关键词	NA+/H+ ANTIPORTER GENE ; SALINITY TOLERANCE ; HKT TRANSPORTERS ; SODIUM-TRANSPORT ; MEMBRANE TRANSPORTERS ; STRESS TOLERANCE ; PLASMA-MEMBRANE ; CELL-DEATH ; K+ EFFLUX ; ARABIDOPSIS
WOS类目	Plant Sciences ; Cell Biology
WOS研究方向	Plant Sciences ; Cell Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217990
作者单位	Inner Mongolia Univ, Coll Life Sci, Minist Educ, Key Lab Herbage & Endem Crop Biotechnol, 24 Zhaojun Rd, Hohhot 010070, Peoples R China
推荐引用方式 GB/T 7714	Li, Ningning,Du, Chao,Ma, Binjie,et al. Functional Analysis of Ion Transport Properties and Salt Tolerance Mechanisms of RtHKT1 from the Recretohalophyte Reaumuria trigyna[J],2019,60(1):85-106.
APA	Li, Ningning.,Du, Chao.,Ma, Binjie.,Gao, Ziqi.,Wu, Zhigang.,...&Wang, Yingchun.(2019).Functional Analysis of Ion Transport Properties and Salt Tolerance Mechanisms of RtHKT1 from the Recretohalophyte Reaumuria trigyna.PLANT AND CELL PHYSIOLOGY,60(1),85-106.
MLA	Li, Ningning,et al."Functional Analysis of Ion Transport Properties and Salt Tolerance Mechanisms of RtHKT1 from the Recretohalophyte Reaumuria trigyna".PLANT AND CELL PHYSIOLOGY 60.1(2019):85-106.