干旱区生态环境知识资源中心(Arid): Contributions of dsRNases to differential RNAi efficiencies between the injection and oral delivery of dsRNA in Locusta migratoria

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DOI	10.1002/ps.5291
	Contributions of dsRNases to differential RNAi efficiencies between the injection and oral delivery of dsRNA in Locusta migratoria
	Song, Huifang 1,3; Fan, Yunhe 1,3; Zhang, Jianqin 1,4; Cooper, Anastasia M. W.2; Silver, Kristopher 2; Li, Daqi 1,5; Li, Tao 1; Ma, Enbo 1; Zhu, Kun Yan 2; Zhang, Jianzhen 1
通讯作者	Zhu, Kun Yan ; Zhang, Jianzhen
来源期刊	PEST MANAGEMENT SCIENCE
ISSN	1526-498X
EISSN	1526-4998
出版年	2019
卷号	75 期号:6 页码:1707-1717
英文摘要	BACKGROUND The efficiency of RNA interference (RNAi) varies considerably among different insect species, and there is growing evidence to suggest that degradation of double-stranded (dsRNA) prior to uptake is an important factor that limits the efficiency of RNAi in insects. In Locusta migratoria, RNAi is highly efficient when dsRNA is delivered by injection, but not by feeding. However, detailed mechanisms causing such differential RNAi efficiency are still elusive. RESULTS We identified and characterized the full-length complementary DNAs (cDNAs) of two new dsRNA nuclease (dsRNase) genes from L. migratoria, which were named LmdsRNase1 and LmdsRNase4. Transcript analyses revealed that LmdsRNase1 and LmdsRNase4 were highly expressed in hemolymph with relatively lower expression in other tested tissues. Our study using heterologously expressed LmdsRNase1 and LmdsRNase4 fusion proteins showed that LmdsRNase1 can degrade dsRNA rapidly at an optimal pH of 5, whereas LmdsRNase4 had no activity at any of the pH values examined. In comparing the substrate specificity of the four LmdsRNases, we found that only LmdsRNase1 and LmdsRNase2 digested dsRNA; however, our experiments suggested that the physiological pH of hemolymph (7.0) suppresses LmdsRNase1 activity permitting significant dsRNA stability in this tissue. Conversely, the physiological pH of midgut juice (6.8) is ideal for LmdsRNase2 activity, resulting in degradation of dsRNA in midgut. CONCLUSION The physiological pH of different insect tissues or compartments can significantly alter the stability of dsRNA by influencing LmdsRNase activity in L. migratoria. Thus, new strategies to overcome such obstacles are expected to help implement RNAi-based technologies for insect pest management. (c) 2018 Society of Chemical Industry
英文关键词	RNA interference dsRNase dsRNA stability RNAi efficiency Locusta migratoria
类型	Article
语种	英语
国家	Peoples R China ; USA
收录类别	SCI-E
WOS记录号	WOS:000468214800024
WOS关键词	DOUBLE-STRANDED-RNA ; DESERT LOCUST ; INSECT PEST ; INTERFERENCE ; NUCLEASE ; EXPRESSION ; ENZYMES ; GUT
WOS类目	Agronomy ; Entomology
WOS研究方向	Agriculture ; Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217935
作者单位	1.Shanxi Univ, Res Inst Appl Biol, Taiyuan 030006, Shanxi, Peoples R China; 2.Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA; 3.Shanxi Univ, Coll Life Sci, Taiyuan, Shanxi, Peoples R China; 4.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan, Shanxi, Peoples R China; 5.Shanxi Acad Agr Sci, Inst Plant Protect, Taiyuan, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Song, Huifang,Fan, Yunhe,Zhang, Jianqin,et al. Contributions of dsRNases to differential RNAi efficiencies between the injection and oral delivery of dsRNA in Locusta migratoria[J],2019,75(6):1707-1717.
APA	Song, Huifang.,Fan, Yunhe.,Zhang, Jianqin.,Cooper, Anastasia M. W..,Silver, Kristopher.,...&Zhang, Jianzhen.(2019).Contributions of dsRNases to differential RNAi efficiencies between the injection and oral delivery of dsRNA in Locusta migratoria.PEST MANAGEMENT SCIENCE,75(6),1707-1717.
MLA	Song, Huifang,et al."Contributions of dsRNases to differential RNAi efficiencies between the injection and oral delivery of dsRNA in Locusta migratoria".PEST MANAGEMENT SCIENCE 75.6(2019):1707-1717.