干旱区生态环境知识资源中心(Arid): Liposome encapsulation and EDTA formulation of dsRNA targeting essential genes increase oral RNAi-caused mortality in the Neotropical stink bug Euschistus heros

Arid

DOI	10.1002/ps.5167
	Liposome encapsulation and EDTA formulation of dsRNA targeting essential genes increase oral RNAi-caused mortality in the Neotropical stink bug Euschistus heros
	Castellanos, Nathaly L.1,2; Smagghe, Guy 1; Sharma, Rohit 1; Oliveira, Eugenio E.2; Christiaens, Olivier 1
通讯作者	Christiaens, Olivier
来源期刊	PEST MANAGEMENT SCIENCE
ISSN	1526-498X
EISSN	1526-4998
出版年	2019
卷号	75 期号:2 页码:537-548
英文摘要	BACKGROUNDThe Neotropical stink bug Euschistus heros is a major pest in soybean fields. Development of highly species-specific pesticides based on RNA interference (RNAi) could provide a new sustainable and environmentally friendly control strategy. RESULTSHere, the potential of RNAi as a pest control tool against E. heros was assessed. First, target gene selection using a microinjection approach was performed. Seven of the 15 candidate genes tested exhibited >95% mortality after hemolymph injection of 27.5ng dsRNA. Subsequently, dsRNA was administered orally using different formulations: naked dsRNA, liposome-encapsulated-dsRNA and dsRNA formulated with EDTA. Liposome-encapsulated dsRNA targeting vATPase A and muscle actin led to significant mortality after 14days (45% and 42%, respectively), whereas EDTA-formulated dsRNA did so for only one of the target genes. Ex vivo analysis of the dsRNA stability in collected saliva indicated a strong dsRNA-degrading capacity by E. heros saliva, which could explain the need for dsRNA formulations. CONCLUSIONThe results demonstrate that continuous ingestion of dsRNA with EDTA or liposome-encapsulated dsRNA can prevent dsRNA from being degraded enzymatically and suggest great potential for using these formulations in dsRNA delivery to use RNAi as a functional genomics tool or for pest management of stink bugs. (c) 2018 Society of Chemical Industry
英文关键词	Euschistus heros RNA interference dsRNA ingestion Lipofectamine (R) 2000 EDTA Hemiptera Pentatomidae
类型	Article
语种	英语
国家	Belgium ; Brazil
收录类别	SCI-E
WOS记录号	WOS:000455406600002
WOS关键词	DOUBLE-STRANDED-RNA ; DIABROTICA-VIRGIFERA-VIRGIFERA ; INTEGRATED PEST-MANAGEMENT ; FUNCTIONAL-CHARACTERIZATION ; BROWN PLANTHOPPER ; MOLECULAR-BASIS ; DESERT LOCUST ; INSECT PEST ; INTERFERENCE ; HEMIPTERA
WOS类目	Agronomy ; Entomology
WOS研究方向	Agriculture ; Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217933
作者单位	1.Univ Ghent, Fac Biosci Engn, Dept Plants & Crops, Coupure Links 653, B-9000 Ghent, Belgium; 2.Univ Fed Vicosa, Dept Entomol, Vicosa, MG, Brazil
推荐引用方式 GB/T 7714	Castellanos, Nathaly L.,Smagghe, Guy,Sharma, Rohit,et al. Liposome encapsulation and EDTA formulation of dsRNA targeting essential genes increase oral RNAi-caused mortality in the Neotropical stink bug Euschistus heros[J],2019,75(2):537-548.
APA	Castellanos, Nathaly L.,Smagghe, Guy,Sharma, Rohit,Oliveira, Eugenio E.,&Christiaens, Olivier.(2019).Liposome encapsulation and EDTA formulation of dsRNA targeting essential genes increase oral RNAi-caused mortality in the Neotropical stink bug Euschistus heros.PEST MANAGEMENT SCIENCE,75(2),537-548.
MLA	Castellanos, Nathaly L.,et al."Liposome encapsulation and EDTA formulation of dsRNA targeting essential genes increase oral RNAi-caused mortality in the Neotropical stink bug Euschistus heros".PEST MANAGEMENT SCIENCE 75.2(2019):537-548.