干旱区生态环境知识资源中心(Arid): Molecular mechanisms influencing efficiency of RNA interference in insects

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DOI	10.1002/ps.5126
	Molecular mechanisms influencing efficiency of RNA interference in insects
	Cooper, Anastasia M. W.1; Silver, Kristopher 1; Zhang, Jianzhen 1,2; Park, Yoonseong 1; Zhu, Kun Yan 1
通讯作者	Zhu, Kun Yan
来源期刊	PEST MANAGEMENT SCIENCE
ISSN	1526-498X
EISSN	1526-4998
出版年	2019
卷号	75 期号:1 页码:18-28
英文摘要	RNA interference (RNAi) is an endogenous, sequence-specific gene-silencing mechanism elicited by small RNA molecules. RNAi is a powerful reverse genetic tool, and is currently being utilized for managing insects and viruses. Widespread implementation of RNAi-based pest management strategies is currently hindered by inefficient and highly variable results when different insect species, strains, developmental stages, tissues, and genes are targeted. Mechanistic studies have shown that double-stranded ribonucleases (dsRNases), endosomal entrapment, deficient function of the core machinery, and inadequate immune stimulation contribute to limited RNAi efficiency. However, a comprehensive understanding of the molecular mechanisms limiting RNAi efficiency remains elusive. Recent advances in dsRNA stability in physiological tissues, dsRNA internalization into cells, the composition and function of the core RNAi machinery, as well as small-interfering RNA/double-stranded RNA amplification and spreading mechanisms are reviewed to establish a global understanding of the obstacles impeding wider understanding of RNAi mechanisms in insects. (c) 2018 Society of Chemical Industry
英文关键词	dsRNA stability dsRNases endocytosis target gene exogenous RNAi systemic RNAi
类型	Review
语种	英语
国家	USA ; Peoples R China
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000452127400004
WOS关键词	DOUBLE-STRANDED-RNA ; SYSTEMIC RNAI ; DESERT LOCUST ; RESISTANCE ; MACHINERY ; EVOLUTION ; PATHWAYS ; GENES ; SIRNA ; BUGS
WOS类目	Agronomy ; Entomology
WOS研究方向	Agriculture ; Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217932
作者单位	1.Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA; 2.Shanxi Univ, Inst Appl Biol, Taiyuan, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Cooper, Anastasia M. W.,Silver, Kristopher,Zhang, Jianzhen,et al. Molecular mechanisms influencing efficiency of RNA interference in insects[J],2019,75(1):18-28.
APA	Cooper, Anastasia M. W.,Silver, Kristopher,Zhang, Jianzhen,Park, Yoonseong,&Zhu, Kun Yan.(2019).Molecular mechanisms influencing efficiency of RNA interference in insects.PEST MANAGEMENT SCIENCE,75(1),18-28.
MLA	Cooper, Anastasia M. W.,et al."Molecular mechanisms influencing efficiency of RNA interference in insects".PEST MANAGEMENT SCIENCE 75.1(2019):18-28.