干旱区生态环境知识资源中心(Arid): Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile

Arid

DOI	10.7717/peerj.7371
	Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile
	Moreno Salas, Lucila 1; Espinoza-Carniglia, Mario 1; Lizama Schmeisser, Nicol 1; Gonzalo Torres, L.1,2; Carolina Silva-de la Fuente, Maria 3,4; Lareschi, Marcela 5; Gonzalez-Acuna, Daniel 3
通讯作者	Moreno Salas, Lucila
来源期刊	PEERJ
ISSN	2167-8359
出版年	2019
卷号	7
英文摘要	Background: Rattus rattus is a widely distributed, invasive species that presents an important role in disease transmission, either directly or through vector arthropods such as fleas. These black rats can transmit a wide variety of pathogens, including bacteria of the genus Bartonella, which can cause diseases in humans and animals. In Chile, no data are available identifying fleas from synanthropic rodents as Bartonella vectors. The aim of this study was to investigate the prevalence of Bartonella spp. in the fleas of R. rattus in areas with different climate conditions and featuring different human population densities. Methods: In all, 174 fleas collected from 261 R. rattus captured from 30 localities with different human densities (cities, villages, and wild areas) across five hydrographic zones of Chile (hyper-arid, arid, semi-arid, sub-humid, and hyper-humid) were examined. Bartonella spp. presence was determined through polymerase chain reaction, using gltA and rpoB genes, which were concatenated to perform a similarity analysis with BLAST and phylogenetic analysis. Results: Overall, 15 fleas species were identified; Bartonella gltA and rpoB fragments were detected in 21.2% (37/174) and 19.5% (34/174) of fleas, respectively. A total of 10 of the 15 fleas species found were positive for Bartonella DNA. Leptopsylla segnis was the most commonly collected flea species (n = 55), and it also presented a high prevalence of Bartonella DNA (P% = 34.5%). The highest numbers of fleas of this species were collected in villages of the arid zone. There were no seasonal differences in the prevalence of Bartonella DNA. The presence of Bartonella DNA in fleas was recorded in all hydrographic areas, and the arid zone presented the highest prevalence of this species. Regarding areas with different human densities, the highest prevalence was noted in the villages (34.8% gltA and 31.8% rpoB), followed by cities (14.8% gltA and 11.1% rpoB) and wild areas (7.4% gltA and 14.8% rpoB). The BLAST analysis showed a high similitude (>96%) with four uncharacterized Bartonella genotypes and with two species with zoonotic potential: B. mastomydis and B. tribocorum. The phylogenetic analysis showed a close relationship with B. elizabethae and B. tribocorum. This is the first study to provide evidence of the presence of Bartonella in fleas of R. rattus in Chile, indicating that the villages and arid zone correspond to areas with higher infection risk.
英文关键词	Infection Infectious diseases Molecular epidemiology Fleas Rodent Chile Anthropogenic effect Public health Diseases Ectoparasites
类型	Article
语种	英语
国家	Chile ; Argentina
开放获取类型	Green Submitted, Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000478561300003
WOS关键词	XENOPSYLLA-CHEOPIS ; SMALL MAMMALS ; MOLECULAR-DETECTION ; SIPHONAPTERA CTENOPHTHALMIDAE ; PHYLOGENETIC ANALYSIS ; SYNANTHROPIC RODENTS ; ZOONOTIC PATHOGENS ; RICKETTSIA-FELIS ; WILD RODENTS ; IDENTIFICATION
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217913
作者单位	1.Univ Concepcion, Fac Ciencias Nat & Oceanog, Dept Zool, Concepcion, Chile; 2.Univ Austral Chile, Fac Ciencias, Programa Magister Ciencias Menc Ecol Aplicada, Valdivia, Chile; 3.Univ Concepcion, Dept Ciencia Anim, Fac Ciencias Vet, Lab Parasitos & Enfermedades Fauna Silvestre, Chillan, Chile; 4.Univ San Sebastian, Fac Med Vet, Concepcion, Chile; 5.UNLP, Ctr Estudios Parasitol & Vectores CEPAVE, CONICET, CCT La Plata, La Plata, Buenos Aires, Argentina
推荐引用方式 GB/T 7714	Moreno Salas, Lucila,Espinoza-Carniglia, Mario,Lizama Schmeisser, Nicol,et al. Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile[J],2019,7.
APA	Moreno Salas, Lucila.,Espinoza-Carniglia, Mario.,Lizama Schmeisser, Nicol.,Gonzalo Torres, L..,Carolina Silva-de la Fuente, Maria.,...&Gonzalez-Acuna, Daniel.(2019).Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile.PEERJ,7.
MLA	Moreno Salas, Lucila,et al."Fleas of black rats (Rattus rattus) as reservoir host of Bartonella spp. in Chile".PEERJ 7(2019).