干旱区生态环境知识资源中心(Arid): Arbuscular mycorrhizal fungi in soil, roots and rhizosphere of Medicago truncatula: diversity and heterogeneity under semi-arid conditions

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DOI	10.7717/peerj.6401
	Arbuscular mycorrhizal fungi in soil, roots and rhizosphere of Medicago truncatula: diversity and heterogeneity under semi-arid conditions
	Mahmoudi, Neji 1; Cruz, Cristina 2; Mahdhi, Mosbah 3; Mars, Mohamed 4; Caeiro, Maria F.4
通讯作者	Caeiro, Maria F.
来源期刊	PEERJ
ISSN	2167-8359
出版年	2019
卷号	7
英文摘要	Mycorrhizal symbioses are considered indicators of ecosystem biodiversity. However, their diversity and relevance in arid and semi-arid ecosystems are poorly understood. This study addressed this subject, the main objective being to evaluate arbuscular mycorrhizal fungi (AMF) diversity and heterogeneity in a semi-arid region. Samples of bulk and rhizosphere soil and fine roots of Medicago truncatula were collected at four different sites with the same aridity index (6.1), in Bou-Hedma National Park, Tunisia, a semi-arid ecosystem. AMF taxa were assessed by 454- pyrosequencing and identified by BLAST matching of operational taxonomic units (OTUs) against the MaarjAM database, targeting AMF SSU rRNA gene diversity. Roots were the hotspots of AMF diversity (107 OTUs out of a total of 138). Of the 138 OTUs, 113 found correspondence in the MaarjAM database, with 32 AMF virtual taxa (VTX), 19 Site-exclusive (SE) and 13 common to at least two sites (Non-site exclusive, NSE); the remaining 25 OTUs grouped in 16 putative new AMF taxa (pNTX), each one consisting of OTUs sharing pairwise distances not higher than 3%. We found a high diversity and heterogeneity of AMF across the four sites, which showed, in a regression analysis, significant relation to six out of the eight environmental parameters evaluated: grazing activity and soil texture, electrical conductivity, organic matter, total phosphorus and total nitrogen. AMF colonization of plants also presented significant differences among the four sites, as well as spore density, microbial biomass and several enzymatic activities (dehydrogenase, beta-glucosidase and phosphatase) evaluated in rhizosphere soils. The four sites clustered in two groups in a hierarchical clustering evaluation based on their AMF diversity (total numbers of OTU, VTX and pNTX) and the parameters referred above. The crucial role of abiotic factors, other than aridity index, on AMF community composition, was evidenced by the high heterogeneity found between AMF communities across sites under identical aridity conditions.
英文关键词	Pyrosequencing Microbial diversity AMF Soil heterogeneity Heterogeneity
类型	Article
语种	英语
国家	Tunisia ; Portugal ; Saudi Arabia
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000460031300001
WOS关键词	HIGH-ALTITUDE ; COMMUNITIES ; IMPACT ; FERTILIZATION ; GLOMEROMYCOTA ; PHYLOGENY ; EVOLUTION ; NITROGEN ; PATTERNS ; RADDIANA
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217900
作者单位	1.Bioressources Zones Arides BVBZA, Unite Rech Biodiversite & Valorisat, Fac Sci Gabes, Erriadh Zrig, Tunisia; 2.Univ Lisbon, Fac Ciencias, Ctr Ecol Evolut & Environm Changes cE3c, Lisbon, Portugal; 3.Jazan Univ, Ctr Environm Res & Studies, Jazan, Saudi Arabia; 4.Univ Lisbon, Fac Ciencias, Ctr Estudos Ambiente & Mar CESAM, Lisbon, Portugal
推荐引用方式 GB/T 7714	Mahmoudi, Neji,Cruz, Cristina,Mahdhi, Mosbah,et al. Arbuscular mycorrhizal fungi in soil, roots and rhizosphere of Medicago truncatula: diversity and heterogeneity under semi-arid conditions[J],2019,7.
APA	Mahmoudi, Neji,Cruz, Cristina,Mahdhi, Mosbah,Mars, Mohamed,&Caeiro, Maria F..(2019).Arbuscular mycorrhizal fungi in soil, roots and rhizosphere of Medicago truncatula: diversity and heterogeneity under semi-arid conditions.PEERJ,7.
MLA	Mahmoudi, Neji,et al."Arbuscular mycorrhizal fungi in soil, roots and rhizosphere of Medicago truncatula: diversity and heterogeneity under semi-arid conditions".PEERJ 7(2019).