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	Bacillus tequilensis ZMS-2: A novel source of alkaline protease with antimicrobial, anti-coagulant, fibrinolytic and dehairing potentials
	Khan, Zahoor 1; Shafique, Maryam 2; Nawaz, Hafiz Rub 3; Jabeen, Nusrat 4; Naz, Sehar Afshan 1
通讯作者	Shafique, Maryam
来源期刊	PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
ISSN	1011-601X
出版年	2019
卷号	32 期号:4 页码:1913-1918
英文摘要	Emerging resistance to existing antimicrobial agents is one of the growing concerns and a serious problem for public health globally. Currently available antimicrobial agents are potent and effective but surfacing resistance to these drugs has not been ruled out so far. Therefore, it is utmost important to explore new bioactive compounds from natural sources to meet future needs. The present study was designed to produce, optimize, characterize and evaluate antimicrobial, fibrinolytic and anti-coagulant potential of a new alkaline protease. Proteolytic strain from desert soils of Tharparkar, Pakistan was subjected to 16S rDNA sequencing and identified as Bacillus tequilensis ZMS-2(Genbank Accession No. MK101013). During submerged fermentation at 37 degrees C, maximum enzyme production (454 U/ml) was observed with 24h old inoculum. The best incubation time was 72h (544 U/ml), optimum inoculum size and pH was 10% at pH 8 with 494 and 506 U/ml, respectively. The best carbon source was starch (571 U/ml), while ideal substrate was wheat bran (536 U/ml). Optimal temperature and pH for proteolytic activity was 60 degrees C (420 U/ml) and 8 (332 U/ml). Alkaline protease showed antibacterial activity against Staphylococcus aureus (27mm), Bacillus licheniformis (20mm), Klebsiella pneumoniae (17mm) and Escherichia coli (15mm). The strain B. tequilensis ZMS-2 also exhibited anti-coagulant, fibrinolytic and dehairing potential suggesting application of its protease in various industries.
英文关键词	Antimicrobial Bacillus tequilensis ZMS-2 alkaline protease anti-coagulant fibrinolytic dehairing
类型	Article
语种	英语
国家	Pakistan
收录类别	SCI-E
WOS记录号	WOS:000485337600025
WOS关键词	EXTRACELLULAR PROTEASE ; ANTICOAGULANT ACTIVITY ; SERINE-PROTEASE ; PURIFICATION ; ENZYME
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217848
作者单位	1.Fed Urdu Univ Arts Sci & Technol, Dept Microbiol, Karachi, Pakistan; 2.Muhammad Ali Jinnah Univ, Dept Biosci, Karachi, Pakistan; 3.Pakistan Council Sci & Ind Res, Leather Res Ctr, D-102, Karachi, Pakistan; 4.Univ Karachi, Dept Microbiol, Karachi, Pakistan
推荐引用方式 GB/T 7714	Khan, Zahoor,Shafique, Maryam,Nawaz, Hafiz Rub,et al. Bacillus tequilensis ZMS-2: A novel source of alkaline protease with antimicrobial, anti-coagulant, fibrinolytic and dehairing potentials[J],2019,32(4):1913-1918.
APA	Khan, Zahoor,Shafique, Maryam,Nawaz, Hafiz Rub,Jabeen, Nusrat,&Naz, Sehar Afshan.(2019).Bacillus tequilensis ZMS-2: A novel source of alkaline protease with antimicrobial, anti-coagulant, fibrinolytic and dehairing potentials.PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,32(4),1913-1918.
MLA	Khan, Zahoor,et al."Bacillus tequilensis ZMS-2: A novel source of alkaline protease with antimicrobial, anti-coagulant, fibrinolytic and dehairing potentials".PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES 32.4(2019):1913-1918.