干旱区生态环境知识资源中心(Arid): De novo Assembly and Annotation of the Blood Transcriptome of the Southern Elephant Seal Mirounga leonina from the South Shetland Islands, Antarctica

Arid

DOI	10.1007/s12601-019-0011-0
	De novo Assembly and Annotation of the Blood Transcriptome of the Southern Elephant Seal Mirounga leonina from the South Shetland Islands, Antarctica
	Kim, Bo-Mi 1; Ahn, Do-Hwan 1; Kang, Seunghyun 1; Jeong, Jihye 1; Jo, Euna 1; Kim, Jeong-Hoon 2; Rhee, Jae-Sung 3,4; Park, Hyun 1,5
通讯作者	Park, Hyun
来源期刊	OCEAN SCIENCE JOURNAL
ISSN	1738-5261
EISSN	2005-7172
出版年	2019
卷号	54 期号:2 页码:307-315
英文摘要	The southern elephant seal Mirounga leonina is the largest member of the phocid seals and is a highly sexually dimorphic predator at circumpolar regions. In this study, we generated the first high-quality de novo assembly of the blood transcriptome of M. leonina using the Illumina MiSeq platform. A total of 40.5 million raw reads were obtained and assembled using the Oases assembly platform, resulting in 46,445 contigs with an N50 value of 634 bp. We performed functional gene annotations through pathway analyses of the Gene Ontology (GO), Eukaryotic Orthologous Groups (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Ortholog comparison revealed that a high proportion of the M. leonina blood transcriptome had significant sequence homology within pinnipeds. M. leonine, a deep and long-diving seal, is routinely exposed to progressive hypoxia during dives. KEGG analyses detected an intact hypoxia-inducible factor-1 (HIF-1) signaling pathway, which is a key metabolism pathway in the adaptive homeostatic responses to hypoxia. In the blood transcriptome, many essential genes involved in innate immunity were detected, which suggests that the blood serves as a host defense modulator against exogenous infections in M. leonina. This genomic resource will be useful for understanding adaptive metabolism upon repeated breath-hold dives and determining the health status of southern elephant seals without the need to sacrifice them in experiments.
英文关键词	Mirounga leonina blood transcriptome hypoxia
类型	Article
语种	英语
国家	South Korea
收录类别	SCI-E
WOS记录号	WOS:000473082100013
WOS关键词	OXIDATIVE STRESS ; EXPRESSION ; INSIGHTS ; PROVIDES
WOS类目	Marine & Freshwater Biology ; Oceanography
WOS研究方向	Marine & Freshwater Biology ; Oceanography
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217801
作者单位	1.KIOST, Korea Polar Res Inst, Unit Polar Genom, Incheon 21990, South Korea; 2.KIOST, Korea Polar Res Inst, Dept Polar Life Sci, Incheon 21990, South Korea; 3.Incheon Natl Univ, Coll Nat Sci, Dept Marine Sci, Incheon 22012, South Korea; 4.Incheon Natl Univ, Res Inst Basic Sci, Incheon 22012, South Korea; 5.Univ Sci Technol, Polar Sci, Daejeon 34113, South Korea
推荐引用方式 GB/T 7714	Kim, Bo-Mi,Ahn, Do-Hwan,Kang, Seunghyun,et al. De novo Assembly and Annotation of the Blood Transcriptome of the Southern Elephant Seal Mirounga leonina from the South Shetland Islands, Antarctica[J],2019,54(2):307-315.
APA	Kim, Bo-Mi.,Ahn, Do-Hwan.,Kang, Seunghyun.,Jeong, Jihye.,Jo, Euna.,...&Park, Hyun.(2019).De novo Assembly and Annotation of the Blood Transcriptome of the Southern Elephant Seal Mirounga leonina from the South Shetland Islands, Antarctica.OCEAN SCIENCE JOURNAL,54(2),307-315.
MLA	Kim, Bo-Mi,et al."De novo Assembly and Annotation of the Blood Transcriptome of the Southern Elephant Seal Mirounga leonina from the South Shetland Islands, Antarctica".OCEAN SCIENCE JOURNAL 54.2(2019):307-315.