Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.1111/1755-0998.13049 |
| Estimating belowground plant abundance with DNA metabarcoding | |
| Matesanz, Silvia1; Pescador, David S.1; Pias, Beatriz2; Sanchez, Ana M.1; Chacon-Labella, Julia3; Illuminati, Angela1; de la Cruz, Marcelino1; Lopez-Angulo, Jesus1; Mari-Mena, Neus4; Vizcaino, Anton4; Escudero, Adrian1 | |
| 通讯作者 | Matesanz, Silvia |
| 来源期刊 | MOLECULAR ECOLOGY RESOURCES
 |
| ISSN | 1755-098X |
| EISSN | 1755-0998 |
| 出版年 | 2019 |
| 卷号 | 19期号:5页码:1265-1277 |
| 英文摘要 | Most work on plant community ecology has been performed above ground, neglecting the processes that occur in the soil. DNA metabarcoding, in which multiple species are computationally identified in bulk samples, can help to overcome the logistical limitations involved in sampling plant communities belowground. However, a major limitation of this methodology is the quantification of species' abundances based on the percentage of sequences assigned to each taxon. Using root tissues of five dominant species in a semi-arid Mediterranean shrubland (Bupleurum fruticescens, Helianthemum cinereum, Linum suffruticosum, Stipa pennata and Thymus vulgaris), we built pairwise mixtures of relative abundance (20%, 50% and 80% biomass), and implemented two methods (linear model fits and correction indices) to improve estimates of root biomass. We validated both methods with multispecies mixtures that simulate field-collected samples. For all species, we found a positive and highly significant relationship between the percentage of sequences and biomass in the mixtures (R-2 = .44-.66), but the equations for each species (slope and intercept) differed among them, and two species were consistently over- and under-estimated. The correction indices greatly improved the estimates of biomass percentage for all five species in the multispecies mixtures, and reduced the overall error from 17% to 6%. Our results show that, through the use of post-sequencing quantification methods on mock communities, DNA metabarcoding can be effectively used to determine not only species' presence but also their relative abundance in field samples of root mixtures. Importantly, knowledge of these aspects will allow us to study key, yet poorly understood, belowground processes. |
| 英文关键词 | coexistence DNA metabarcoding Mediterranean shrubland mock communities plant abundance rbcL region root biomass sequence |
| 类型 | Article |
| 语种 | 英语 |
| 国家 | Spain |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000477303100001 |
| WOS关键词 | SPECIES ABUNDANCE ; SPATIAL-PATTERNS ; SOIL FEEDBACKS ; SINGLE ; ROOTS ; BIODIVERSITY ; COMMUNITIES ; RICHNESS ; BARCODES ; BIAS |
| WOS类目 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/217655 |
| 作者单位 | 1.Univ Rey Juan Carlos, Area Biodiversidad & Conservac, Mostoles, Spain; 2.Univ Complutense Madrid, Dept Biodiversidad Ecol & Evoluc, Madrid, Spain; 3.Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Dept Medio Ambiente, Madrid, Spain; 4.AllGenet & Biol SL, Edificio CICA, Coruna, Spain |
| 推荐引用方式 GB/T 7714 | Matesanz, Silvia,Pescador, David S.,Pias, Beatriz,et al. Estimating belowground plant abundance with DNA metabarcoding[J],2019,19(5):1265-1277. |
| APA | Matesanz, Silvia.,Pescador, David S..,Pias, Beatriz.,Sanchez, Ana M..,Chacon-Labella, Julia.,...&Escudero, Adrian.(2019).Estimating belowground plant abundance with DNA metabarcoding.MOLECULAR ECOLOGY RESOURCES,19(5),1265-1277. |
| MLA | Matesanz, Silvia,et al."Estimating belowground plant abundance with DNA metabarcoding".MOLECULAR ECOLOGY RESOURCES 19.5(2019):1265-1277. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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