干旱区生态环境知识资源中心(Arid): Plant DNA-barcode library and community phylogeny for a semi-arid East African savanna

Arid

DOI	10.1111/1755-0998.13001
	Plant DNA-barcode library and community phylogeny for a semi-arid East African savanna
	Gill, Brian A.1; Musili, Paul M.2; Kurukura, Samson 3; Hassan, Abidikadir A.3; Goheen, Jacob R.4,5; Kress, W. John 6; Kuzmina, Maria 7; Pringle, Robert M.8; Kartzinel, Tyler R.1,9
通讯作者	Kartzinel, Tyler R.
来源期刊	MOLECULAR ECOLOGY RESOURCES
ISSN	1755-098X
EISSN	1755-0998
出版年	2019
卷号	19 期号:4 页码:838-846
英文摘要	Applications of DNA barcoding include identifying species, inferring ecological and evolutionary relationships between species, and DNA metabarcoding. These applications require reference libraries that are not yet available for many taxa and geographic regions. We collected, identified, and vouchered plant specimens from Mpala Research Center in Laikipia, Kenya, to develop an extensive DNA-barcode library for a savanna ecosystem in equatorial East Africa. We amassed up to five DNA barcode markers (rbcL, matK, trnL-F, trnH-psbA, and ITS) for 1,781 specimens representing up to 460 species (92% of the known flora), increasing the number of plant DNA barcode records for Africa by 9%. We evaluated the ability of these markers, singly and in combination, to delimit species by calculating intra- and interspecific genetic distances. We further estimated a plant community phylogeny and demonstrated its utility by testing if evolutionary relatedness could predict the tendency of members of the Mpala plant community to have or lack barcode gaps, defined as disparities between the maximum intra- and minimum interspecific genetic distances. We found barcode gaps for 72%-89% of taxa depending on the marker or markers used. With the exception of the markers rbcL and ITS, we found that evolutionary relatedness was an important predictor of barcode-gap presence or absence for all of the markers in combination and for matK, trnL-F, and trnH-psbA individually. This plant DNA barcode library and community phylogeny will be a valuable resource for future investigations.
英文关键词	barcode gap biodiversity comparative phylogenetics East Africa Forest Global Earth Observatory Mpala Research Centre
类型	Article
语种	英语
国家	USA ; Kenya ; Canada
收录类别	SCI-E
WOS记录号	WOS:000471830200004
WOS关键词	SUBFAMILY ALOOIDEAE ; SOUTHERN AFRICA ; SEQUENCE DATA ; ACACIA-S.L. ; COMBRETACEAE ; HERBIVORES ; DIVERSITY ; PATTERNS ; FOREST ; DIVERSIFICATION
WOS类目	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217654
作者单位	1.Brown Univ, Inst Environm & Soc, 85 Waterman St, Providence, RI 02912 USA; 2.Natl Museums Kenya, East African Herbarium, Nairobi, Kenya; 3.Mpala Res Ctr, Nanyuki, Kenya; 4.Univ Wyoming, Dept Zool, Laramie, WY 82071 USA; 5.Univ Wyoming, Dept Physiol, Laramie, WY 82071 USA; 6.Smithsonian Inst, Natl Museum Nat Hist, Washington, DC 20560 USA; 7.Univ Guelph, Ctr Biodivers Genom, Guelph, ON, Canada; 8.Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA; 9.Brown Univ, Dept Ecol & Evolutionary Biol, Providence, RI 02912 USA
推荐引用方式 GB/T 7714	Gill, Brian A.,Musili, Paul M.,Kurukura, Samson,et al. Plant DNA-barcode library and community phylogeny for a semi-arid East African savanna[J],2019,19(4):838-846.
APA	Gill, Brian A..,Musili, Paul M..,Kurukura, Samson.,Hassan, Abidikadir A..,Goheen, Jacob R..,...&Kartzinel, Tyler R..(2019).Plant DNA-barcode library and community phylogeny for a semi-arid East African savanna.MOLECULAR ECOLOGY RESOURCES,19(4),838-846.
MLA	Gill, Brian A.,et al."Plant DNA-barcode library and community phylogeny for a semi-arid East African savanna".MOLECULAR ECOLOGY RESOURCES 19.4(2019):838-846.