干旱区生态环境知识资源中心(Arid): Safe Cultivation of Medicago sativa in Metal-Polluted Soils from Semi-Arid Regions Assisted by Heat- and Metallo-Resistant PGPR

Arid

DOI	10.3390/microorganisms7070212
	Safe Cultivation of Medicago sativa in Metal-Polluted Soils from Semi-Arid Regions Assisted by Heat- and Metallo-Resistant PGPR
	Raklami, Anas 1,2,3; Oufdou, Khalid 1; Tahiri, Abdel-Ilah 1,2; Mateos-Naranjo, Enrique 4; Navarro-Torre, Salvadora 3; Rodriguez-Llorente, Ignacio D.3; Meddich, Abdelilah 2; Redondo-Gomez, Susana 4; Pajuelo, Eloisa 3
通讯作者	Pajuelo, Eloisa
来源期刊	MICROORGANISMS
EISSN	2076-2607
出版年	2019
卷号	7 期号:7
英文摘要	Soil contamination with heavy metals is a constraint for plant establishment and development for which phytoremediation may be a solution, since rhizobacteria may alleviate plant stress under these conditions. A greenhouse experiment was conducted to elucidate the effect of toxic metals on growth, the activities of ROS (reactive oxygen species)-scavenging enzymes, and gene expression of Medicago sativa grown under different metal and/or inoculation treatments. The results showed that, besides reducing biomass, heavy metals negatively affected physiological parameters such as chlorophyll fluorescence and gas exchange, while increasing ROS-scavenging enzyme activities. Inoculation of M. sativa with a bacterial consortium of heat- and metallo-resistant bacteria alleviated metal stress, as deduced from the improvement of growth, lower levels of antioxidant enzymes, and increased physiological parameters. The bacteria were able to effectively colonize and form biofilms onto the roots of plants cultivated in the presence of metals, as observed by scanning electron microscopy. Results also evidenced the important role of glutathione reductase (GR), phytochelatin synthase (PCS), and metal transporter NRAMP1 genes as pathways for metal stress management, whereas the gene coding for cytochrome P450 (CP450) seemed to be regulated by the presence of the bacteria. These outcomes showed that the interaction of metal-resistant rhizobacteria/legumes can be used as an instrument to remediate metal-contaminated soils, while cultivation of inoculated legumes on these soils is still safe for animal grazing, since inoculation with bacteria diminished the concentrations of heavy metals accumulated in the aboveground parts of the plants to below toxic levels.
英文关键词	heavy metal stress PGPR rhizobia legumes stress related genes ROS-scavenging enzymes biofilms scanning electron microscopy
类型	Article
语种	英语
国家	Morocco ; Spain
开放获取类型	gold, Green Published, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000478618800014
WOS关键词	PLANT-GROWTH ; CONTAMINATED SOILS ; OXIDATIVE STRESS ; PHYTOREMEDIATION ; BACTERIA ; PHOTOSYNTHESIS ; ACCUMULATION ; REMEDIATION ; EXPRESSION ; COPPER
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217611
作者单位	1.Cadi Ayyad Univ, Fac Sci Semlalia, Lab Biol & Biotechnol Microorganisms, POB 2390, Marrakech, Morocco; 2.Cadi Ayyad Univ, Fac Sci Semlalia, Lab Biotechnol & Plant Physiol, POB 2390, Marrakech, Morocco; 3.Univ Seville, Dept Microbiol & Parasitol, POB 1095, Seville 41080, Spain; 4.Univ Seville, Dept Plant Biol & Ecol, POB 1095, Seville 41080, Spain
推荐引用方式 GB/T 7714	Raklami, Anas,Oufdou, Khalid,Tahiri, Abdel-Ilah,et al. Safe Cultivation of Medicago sativa in Metal-Polluted Soils from Semi-Arid Regions Assisted by Heat- and Metallo-Resistant PGPR[J],2019,7(7).
APA	Raklami, Anas.,Oufdou, Khalid.,Tahiri, Abdel-Ilah.,Mateos-Naranjo, Enrique.,Navarro-Torre, Salvadora.,...&Pajuelo, Eloisa.(2019).Safe Cultivation of Medicago sativa in Metal-Polluted Soils from Semi-Arid Regions Assisted by Heat- and Metallo-Resistant PGPR.MICROORGANISMS,7(7).
MLA	Raklami, Anas,et al."Safe Cultivation of Medicago sativa in Metal-Polluted Soils from Semi-Arid Regions Assisted by Heat- and Metallo-Resistant PGPR".MICROORGANISMS 7.7(2019).