干旱区生态环境知识资源中心(Arid): Screening and quantitation of forty-six drugs of abuse and toxic compounds in human whole blood by capillary electrophoresis: Application to forensic cases

Arid

DOI	10.1016/j.microc.2018.09.031
	Screening and quantitation of forty-six drugs of abuse and toxic compounds in human whole blood by capillary electrophoresis: Application to forensic cases
	Cui, Xuezi 1,2; Ni, Chunfang 2; Liang, Chen 2; Gong, Feijun 2; Wang, Rong 2; Chen, Guiliang 3; Zhang, Yurong 2
通讯作者	Chen, Guiliang ; Zhang, Yurong
来源期刊	MICROCHEMICAL JOURNAL
ISSN	0026-265X
EISSN	1095-9149
出版年	2019
卷号	144 页码:403-410
英文摘要	A novel capillary electrophoresis method, coupled with photo-diode array detection, was established for the analysis of forty-six drugs of abuse, including opiates, cocaine, amphetamines, benzodiazepines, and alkaloids, in human whole blood. Doxapram was used as the internal standard, and an Oasis HLB column was used for solid phase extraction of drugs from human whole blood. This method was applied to analyze real blood samples in forensic cases. The separation was performed on a fused-silica capillary (75 mu m I.D. x 50.2 cm, with 40 cm of effect length). In this study, the running buffer was 150 mM phosphate buffer (pH 2.4) containing 20% methanol. Samples were injected at a pressure of 0.5 psi for 10 s and separated at a running voltage of 16 kV. The precision, accuracy, sensitivity, linear range, limit of detection, and recovery of the method were evaluated. The time taken for the entire separation process was 18.5 min. All compounds were identified by relative retention times, and the peak height ratios (the ratio between the target analyte peak height and the internal standard peak height) were monitored at 200 nm and 210 nm in corresponding ultraviolet spectra. 210 nm was used as quantitative detection wavelength. Calibration curves were established for all compounds of interest For human blood samples, the detection limits were 8-80 ng/mL, and the linear range was 0.03-10 pg/mL. The intraday and interday precisions were both less than 13%. This method offers simple and effective screening and quantitation of drugs of abuse and toxic compounds in human blood at toxicologically relevant concentrations, and was applied to analyze blood samples from forensic cases.
英文关键词	Capillary electrophoresis Solid phase extraction Abuse and toxic compounds Human whole blood Screening and quantitation
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E ; SSCI
WOS记录号	WOS:000449893000054
WOS关键词	FLIGHT MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; AMPHETAMINE-TYPE STIMULANTS ; ZONE-ELECTROPHORESIS ; PHARMACEUTICAL-PREPARATIONS ; CHIRAL SEPARATIONS ; HUMAN PLASMA ; HUMAN URINE ; GC-MS
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217605
作者单位	1.China State Inst Pharmaceut Ind, Shanghai, Peoples R China; 2.Shanghai Inst Forens Sci, Shanghai Key Lab Crime Scene Evidence, 803 Zhong Shan Bei Yi Rd, Shanghai 200083, Peoples R China; 3.Shanghai Ctr Drug Evaluat & Inspect, 781 Cai Lun Rd, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Cui, Xuezi,Ni, Chunfang,Liang, Chen,et al. Screening and quantitation of forty-six drugs of abuse and toxic compounds in human whole blood by capillary electrophoresis: Application to forensic cases[J],2019,144:403-410.
APA	Cui, Xuezi.,Ni, Chunfang.,Liang, Chen.,Gong, Feijun.,Wang, Rong.,...&Zhang, Yurong.(2019).Screening and quantitation of forty-six drugs of abuse and toxic compounds in human whole blood by capillary electrophoresis: Application to forensic cases.MICROCHEMICAL JOURNAL,144,403-410.
MLA	Cui, Xuezi,et al."Screening and quantitation of forty-six drugs of abuse and toxic compounds in human whole blood by capillary electrophoresis: Application to forensic cases".MICROCHEMICAL JOURNAL 144(2019):403-410.