干旱区生态环境知识资源中心(Arid): Active microbiome structure and its association with environmental factors and viruses at different aquatic sites of a high-altitude wetland

Arid

DOI	10.1002/mbo3.667
	Active microbiome structure and its association with environmental factors and viruses at different aquatic sites of a high-altitude wetland
	Eissler, Yoanna 1; Galvez, Maria-Jesus 2,3; Dorador, Cristina 4,5; Hengst, Martha 5,6; Molina, Veronica
通讯作者	Molina, Veronica
来源期刊	MICROBIOLOGYOPEN
ISSN	2045-8827
出版年	2019
卷号	8 期号:3
英文摘要	Salar de Huasco is a high-altitude wetland characterized by a highly diverse microbial life adapted to extreme climatic and environmental conditions. Our study aims to determine active microbial community structure changes within different aquatic sites and its relationship with environmental factors and viruses as potential drivers of diversification in different aquatic areas of this ecosystem. In this study, bacteria and archaea composition (16S rRNA subunit pyrolibraries) and picoplankton and viral abundance were determined at ponds, springs and lagoon sites of the wetland during wet and dry seasons (February and July 2012, respectively). In general, mixosaline waters (1,400-51,000 mu S/cm) usually found in ponds arid lagoon presented higher picoplanktonic abundances compared to freshwater (<800 mu S/cm) spring sites, ranging from 1.07 x 10(5) to 1.83 x 10(7) cells/ml. Viral abundance and viral to picoplankton ratio (VPR) also presented greater values at ponds compared to spring sites, reaching up to 4.78 x 10(8) viruses-like particles and up to 351 for VPR. In general, ponds hold a higher microbial diversity and complexity associated also with the presence of microbial mats compared with water sources or lagoon (Shannon index H' 2.6-3,9 vs. <2.0). A greater richness of archaea was also detected in ponds characterized by functional groups such as known methanogens and ammonia oxidizers, and uncultured groups. In total, our results indicate that among the different aquatic sites of the wetland, ponds presented a great microbial community diversification associated to a higher top-down control by viruses which may influence nutrient and greenhouse gases cycling.
英文关键词	16S rRNA subunit archaea bacteria high altitude wetland picoplankton virus
类型	Article
语种	英语
国家	Chile
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000463972500021
WOS关键词	TOP-DOWN ; FRESH-WATER ; BOTTOM-UP ; COMMUNITY STRUCTURE ; FLOW-CYTOMETRY ; DIVERSITY ; ARCHAEA ; MARINE ; VIRIOPLANKTON ; SEDIMENT
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217604
作者单位	1.Univ Valparaiso, Fac Ciencias, Ctr Invest & Gest Recursos Nat, Inst Quim & Bioquim, Valparaiso, Chile; 2.Univ Playa Ancha, Programa Biodiversidad, Fac Ciencias Nat & Exactas, Observ Ecol Microbiana, Valparaiso, Chile; 3.Univ Playa Ancha, Dept Biol, Fac Ciencias Nat & Exactas, Observ Ecol Microbiana, Valparaiso, Chile; 4.Univ Antofagasta, Lab Complejidad Microbiana & Ecol Func, Dept Biotecnol, Fac Ciencias Mar & Recursos Biol, Antofagasta, Chile; 5.Ctr Biotechnol & Bioengn, Santiago, Chile; 6.Univ Catolica Norte, Dept Ciencias Farmaceut, Fac Ciencias, Antofagasta, Chile
推荐引用方式 GB/T 7714	Eissler, Yoanna,Galvez, Maria-Jesus,Dorador, Cristina,et al. Active microbiome structure and its association with environmental factors and viruses at different aquatic sites of a high-altitude wetland[J],2019,8(3).
APA	Eissler, Yoanna,Galvez, Maria-Jesus,Dorador, Cristina,Hengst, Martha,&Molina, Veronica.(2019).Active microbiome structure and its association with environmental factors and viruses at different aquatic sites of a high-altitude wetland.MICROBIOLOGYOPEN,8(3).
MLA	Eissler, Yoanna,et al."Active microbiome structure and its association with environmental factors and viruses at different aquatic sites of a high-altitude wetland".MICROBIOLOGYOPEN 8.3(2019).