干旱区生态环境知识资源中心(Arid): Culture-independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil-bioremediation

Arid

DOI	10.1002/mbo3.630
	Culture-independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil-bioremediation
	Dashti, Narjes; Ali, Nedaa; Salamah, Samar; Khanafer, Majida; Al-Shamy, Ghada; Al-Awadhi, Husain; Radwan, Samir S.
通讯作者	Radwan, Samir S.
来源期刊	MICROBIOLOGYOPEN
ISSN	2045-8827
出版年	2019
卷号	8 期号:2
英文摘要	To analyze microbial communities in environmental samples, this study combined Denaturing Gradient Gel Electrophoresis of amplified 16S rRNA-genes in total genomic DNA extracts from those samples with gene sequencing. The environmental samples studied were oily seawater and soil samples, that had been bioaugmented with natural materials rich in hydrocarbonoclastic bacteria. This molecular approach revealed much more diverse bacterial taxa than the culture-dependent method we had used in an earlier study for the analysis of the same samples. The study described the dynamics of bacterial communities during bioremediation. The main limitation associated with this molecular approach, namely of not distinguishing hydrocarbonoclastic taxa from others, was overcome by consulting the literature for the hydrocarbonoclastic potential of taxa related to those identified in this study. By doing so, it was concluded that the hydrocarbonoclastic bacterial taxa were much more diverse than those captured by the culture-dependent approach. The molecular analysis also revealed the frequent occurrence of nifH-genes in the total genomic DNA extracts of all the studied environmental samples, which reflects a nitrogen-fixation potential. Nitrogen fertilization is long known to enhance microbial oil-bioremediation. The study revealed that bioaugmentation using plant rhizospheres or soil with long history of oil-pollution was more effective in oil-removal in the desert soil than in seawater microcosms.
英文关键词	bioremediation hydrocarbonoclastic bacteria molecular analysis nifH-genes oil spills
类型	Article
语种	英语
国家	Kuwait
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000459746700003
WOS关键词	AUTOCHTHONOUS BIOAUGMENTATION ; CONTAMINATED SOIL ; NITROGEN-FIXATION ; DIESEL-OIL ; RHIZOSPHERE ; FEASIBILITY ; DEGRADATION ; POPULATIONS ; GENES
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217603
作者单位	Kuwait Univ, Fac Sci, Dept Biol Sci, Microbiol Program, Safat, Kuwait
推荐引用方式 GB/T 7714	Dashti, Narjes,Ali, Nedaa,Salamah, Samar,et al. Culture-independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil-bioremediation[J],2019,8(2).
APA	Dashti, Narjes.,Ali, Nedaa.,Salamah, Samar.,Khanafer, Majida.,Al-Shamy, Ghada.,...&Radwan, Samir S..(2019).Culture-independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil-bioremediation.MICROBIOLOGYOPEN,8(2).
MLA	Dashti, Narjes,et al."Culture-independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil-bioremediation".MICROBIOLOGYOPEN 8.2(2019).