干旱区生态环境知识资源中心(Arid): Determination of antioxidant capacity, phenolic acid composition and antiproliferative effect associated with phenylalanine ammonia lyase (PAL) activity in some plants naturally growing under salt stress

Arid

DOI	10.1007/s00044-018-2278-6
	Determination of antioxidant capacity, phenolic acid composition and antiproliferative effect associated with phenylalanine ammonia lyase (PAL) activity in some plants naturally growing under salt stress
	Sirin, Seda; Aslim, Belma
通讯作者	Sirin, Seda
来源期刊	MEDICINAL CHEMISTRY RESEARCH
ISSN	1054-2523
EISSN	1554-8120
出版年	2019
卷号	28 期号:3 页码:229-238
英文摘要	Being a considerable abiotic stress factor particularly for arid and semi-arid regions, salt stress may significantly limit the plant growth and yield. Plant's response to salt stress involves secondary metabolites and especially phenylpropanoids that significantly contribute to the antioxidant activity of plant tissues. In addition to their important role in the control of cancer, phenylpropanoid compounds act as quenchers of singlet oxygen formation, free radical scavengers and reducing agents. One of the important gateway enzymes in the secondary metabolic pathway leading to the synthesis of phenylpropanoids is phenylalanine ammonia lyase (PAL). The aim of this study is to determine the phenolic acid composition, antioxidant capacity and antiproliferative effect associated with PAL activity in some plants that grow naturally under salt stress. The PAL activities of Salsola nitraria, Salvia halophila and Cyathobasis fruticulosa were evaluated. The antioxidant content of the extracts was studied and they were evaluated for their antioxidant activity. MTT assay was used to determine the antiproliferative effects of the extracts on HT-29 cells. Also, phenolic acids in extracts, namely p-coumaric acid, vanillic acid, gallic acid, caffeic acid, chlorogenic acid and syringic acid were screened using LC-MS/MS. Considering all results, C. fruticulosa with its highest PAL activity (62.85mol.min(-1).mg(-1) protein) has become prominent among the three plants. C. fruticulosa extract exhibited the highest antioxidant content with total phenolic content (120.36mg/g) as the major antioxidant component. It was also found to be the plant extract richest in p-coumaric acid, vanillic acid, gallic acid, caffeic acid, chlorogenic acid and syringic acid as phenolic acids. Besides its marked antiproliferative activity against HT-29 cells, C. fruticulosa extract had the highest antioxidant activity compared with other extracts. In conclusion, the antioxidant and anticancer properties of plants naturally growing under salt stress may partly arise from their high PAL activities. Therefore, compounds obtained through plants exhibiting high levels of PAL activities could be used in the development of new pharmaceuticals as an antioxidant and anticancer agent.
英文关键词	Antioxidant Antiproliferative Phenylalanine ammonia lyase (PAL) Phenolic acid Salt stress
类型	Article
语种	英语
国家	Turkey
收录类别	SCI-E
WOS记录号	WOS:000459449600001
WOS关键词	VANILLIC ACID ; SALINITY STRESS ; CAFFEIC ACID ; GALLIC ACID ; L. ; FLAVONOIDS ; PEROXIDATION ; CAROTENOIDS ; DERIVATIVES ; OXIDATION
WOS类目	Chemistry, Medicinal
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217570
作者单位	Gazi Univ, Dept Biol, Fac Sci, TR-06500 Ankara, Turkey
推荐引用方式 GB/T 7714	Sirin, Seda,Aslim, Belma. Determination of antioxidant capacity, phenolic acid composition and antiproliferative effect associated with phenylalanine ammonia lyase (PAL) activity in some plants naturally growing under salt stress[J],2019,28(3):229-238.
APA	Sirin, Seda,&Aslim, Belma.(2019).Determination of antioxidant capacity, phenolic acid composition and antiproliferative effect associated with phenylalanine ammonia lyase (PAL) activity in some plants naturally growing under salt stress.MEDICINAL CHEMISTRY RESEARCH,28(3),229-238.
MLA	Sirin, Seda,et al."Determination of antioxidant capacity, phenolic acid composition and antiproliferative effect associated with phenylalanine ammonia lyase (PAL) activity in some plants naturally growing under salt stress".MEDICINAL CHEMISTRY RESEARCH 28.3(2019):229-238.