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DOI	10.1016/j.lwt.2018.10.052
	Determination of phenolic compounds and antioxidant activity in passion fruit pulp (Passiflora spp.) using a modified QuEChERS method and UHPLC-MS/MS
	Rotta, Eliza Mariane 1; Rodrigues, Carina Alexandra 1; Sales Fontes Jardim, Isabel Cristina 2; Maldaner, Liane 1; Visentainer, Jesui Vergilio 1
通讯作者	Visentainer, Jesui Vergilio
来源期刊	LWT-FOOD SCIENCE AND TECHNOLOGY
ISSN	0023-6438
EISSN	1096-1127
出版年	2019
卷号	100 页码:397-403
英文摘要	A new method based on the modified QuEChERS approach was developed for determination of phenolic compounds in passion fruit pulp (Passifiora spp.) using UHPLC-MS/MS. Studies of extraction/partitioning and cleanup were carried out using different variations of the QuEChERS method (original, acetate, and citrate) and cleanup sorbents (PSA, GCB, C18 and Oasis(center dot) HLB). The original QuEChERS method with 25 mg of C18 as dispersive cleaning sorbent showed the best results for the proposed phenolic compounds (quercetin, rutin, 4-hydroxybenzoic, chlorogenic, ferulic, vanillic, caffeic, trans-cinammic, and p-coumaric acids). Under the optimized conditions, validation presented acceptable results for linearity (r(2) >= 0.99), precision (RSD <= 20%), recovery (73-115%), and the limits of quantification (4-80 mu g/kg). The method developed was successfully applied to different species, including Passiflora edulis, Passifiora alata, and Passifiora ligularis. P. edulis was the only species that contained all target phenolic compounds, with the highest abundance of vanillic acid (426 +/- 29 mu g/kg) and quercetin (416 +/- 6 mu g/kg). In P. alata and P. ligularis species, rutin (289 +/- 6 mu g/kg) and caffeic acid (64 +/- 2 mu g/kg), respectively, were found at the highest levels. In addition, total phenolic content and antioxidant activity assays (DPPH radical and ABTS cation radical assays) correlated with the UHPLC-MS/MS analysis for all passion fruit pulps analyzed.
英文关键词	Yellow passion fruit Sweet passion fruit Granadilla
类型	Article
语种	英语
国家	Brazil
收录类别	SCI-E
WOS记录号	WOS:000452946300056
WOS关键词	SOLID-PHASE EXTRACTION ; PESTICIDE-RESIDUES
WOS类目	Food Science & Technology
WOS研究方向	Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217518
作者单位	1.State Univ Maringa UEM, Chem Dept, Maringa, Parana, Brazil; 2.Univ Estadual Campinas, Inst Chem, UNICAMP, Campinas, SP, Brazil
推荐引用方式 GB/T 7714	Rotta, Eliza Mariane,Rodrigues, Carina Alexandra,Sales Fontes Jardim, Isabel Cristina,et al. Determination of phenolic compounds and antioxidant activity in passion fruit pulp (Passiflora spp.) using a modified QuEChERS method and UHPLC-MS/MS[J],2019,100:397-403.
APA	Rotta, Eliza Mariane,Rodrigues, Carina Alexandra,Sales Fontes Jardim, Isabel Cristina,Maldaner, Liane,&Visentainer, Jesui Vergilio.(2019).Determination of phenolic compounds and antioxidant activity in passion fruit pulp (Passiflora spp.) using a modified QuEChERS method and UHPLC-MS/MS.LWT-FOOD SCIENCE AND TECHNOLOGY,100,397-403.
MLA	Rotta, Eliza Mariane,et al."Determination of phenolic compounds and antioxidant activity in passion fruit pulp (Passiflora spp.) using a modified QuEChERS method and UHPLC-MS/MS".LWT-FOOD SCIENCE AND TECHNOLOGY 100(2019):397-403.