干旱区生态环境知识资源中心(Arid): Application of Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy in the Screening of Strains Producing Bioactive Molecules: A Metabolomics Approach

Arid

	Application of Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy in the Screening of Strains Producing Bioactive Molecules: A Metabolomics Approach
	Boumehira, A. Z.1,2,3; Arous, O.2; Elsayed, E. A.4,5; Hacene, H.2; Yezli, N.3; Sukmawati, D.6; El-Enshasy, H. A.7,8
通讯作者	Elsayed, E. A.
来源期刊	JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH
ISSN	0022-4456
EISSN	0975-1084
出版年	2019
卷号	78 期号:5 页码:301-306
英文摘要	The Recent developments in infrared spectroscopy have made it a method of choice for metabolomics study as it provides accurate analysis with low operating costs due to availability of spectra processing software and chemometrics analysis. The aim of this work is to demonstrate the high potential application of Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy (ATR/FTIR) in rapid screening of microbial strains producing bioactive molecules. In this work, 101 strains were isolated from soil samples collected from the Algerian Sahara desert. ATR/FTIR spectroscopy was applied to eliminate replicate strains. After initial screening, the obtained spectra were divided into two regions. The first, in region between 4000-2250 cm(-1), contain C-H bond stretching vibrations of methyl and methylene of fatty acid membranes, which is very helpful in discriminating Gram positive from Gram negative bacteria. The second region, between 1800-900 cm(-1) (amides I and II), 1800-1500 cm(-1)(peptides and proteins), and 960 cm(-1) (nucleic acids) were observed. Applying this method as first step in screening enabled us to reduce the sample number by 71.28%. Combining this technique with antibacterial test of antagonism, further reductionin strain number by 87.12% was obtained. Accordingly, total screening costs as well as time for bioactive metabolite screening program were reduced.
英文关键词	ATR/FTIR Spectroscopy Bioactive metabolites Metabolome Rapid screening
类型	Article
语种	英语
国家	Algeria ; Saudi Arabia ; Egypt ; Indonesia ; Malaysia
收录类别	SCI-E
WOS记录号	WOS:000469893900008
WOS关键词	NATAMYCIN PRODUCTION ; ANTIBIOTICS
WOS类目	Engineering, Multidisciplinary
WOS研究方向	Engineering
来源机构	King Saud University
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217315
作者单位	1.Univ Algiers, Fac Sci, Algiers, Algeria; 2.Univ Sci & Technol Houari Boumediene, Algiers 16111, Algeria; 3.Ctr Rech Sci & Tech Anal Physico Chim, Bou Ismail 42004, Tipaza, Algeria; 4.King Saud Univ, Coll Sci, Dept Zool, Bioprod Res Chair, Riyadh 11451, Saudi Arabia; 5.Natl Res Ctr, Nat & Microbial Prod Dept, Cairo 12311, Egypt; 6.Univ Negeri Jakarta, Fac Math & Nat Sci, Dept Biol, Jakarta, Indonesia; 7.UTM, Inst Bioprod Dev, Johor Baharu 81310, Malaysia; 8.City Sci Res & Technol Applicat, Alexandria 21934, Egypt
推荐引用方式 GB/T 7714	Boumehira, A. Z.,Arous, O.,Elsayed, E. A.,et al. Application of Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy in the Screening of Strains Producing Bioactive Molecules: A Metabolomics Approach[J]. King Saud University,2019,78(5):301-306.
APA	Boumehira, A. Z..,Arous, O..,Elsayed, E. A..,Hacene, H..,Yezli, N..,...&El-Enshasy, H. A..(2019).Application of Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy in the Screening of Strains Producing Bioactive Molecules: A Metabolomics Approach.JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH,78(5),301-306.
MLA	Boumehira, A. Z.,et al."Application of Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy in the Screening of Strains Producing Bioactive Molecules: A Metabolomics Approach".JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH 78.5(2019):301-306.