干旱区生态环境知识资源中心(Arid): Enantiomeric determination of alpha-lipoic acid in urine by LC/MS/MS

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DOI	10.1016/j.jpba.2019.01.042
	Enantiomeric determination of alpha-lipoic acid in urine by LC/MS/MS
	Kobayashi, Yoshiyuki; Ito, Rie; Saito, Koichi
通讯作者	Saito, Koichi
来源期刊	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN	0731-7085
EISSN	1873-264X
出版年	2019
卷号	166 页码:435-439
英文摘要	An analytical method for the enantiomeric determination of alpha-lipoic acid in human urine was developed to evaluate the pharmacokinetics of alpha-lipoic acid, an ingredient in health food. Urine samples were collected over time after administering alpha-lipoic acid dietary supplement to healthy subjects. The samples were cleaned up by solid-phase extraction using an Oasis (R) MAX cartridge. In the LC/MS/MS method, CHI-RALPAK AD-3R was used as the chiral separation column and acetonitrile-methanol-formic acid (10 mM) (25:25:50, v/v/v) was used as the mobile phase. C-13(4) 1,2,5,6-d-l-alpha-Lipoic acid was used as the internal standard. MS/MS was performed by electrospray ionization (ESI) in the negative ion mode using two monitoring ion transitions (m/z 205.0 -> 170.9 and m/z 209.0 -> 174.9). A calibration curve was prepared in the concentration range of 0.5-100 ng/mL for each enantiomer (r > 0.9999). The limit of detection (LOD, S/N = 3) and the limit of quantification (LOQ S/N > 10) were 0.1 ng/mL and 0.5 ng/mL, respectively. The intra-day and inter-day accuracy of alpha-lipoic acid enantiomers at the LOQ level (0.5 ng/mL), the low concentration level (5 ng/mL), the middle concentration level (50 ng/mL), and the high concentration level (100 ng/mL) ranged from 93.7 to 103.1%. The intra-day and inter-day precision were <= 6.94% and <= 7.05%, respectively. Calculating the mean values of pharmacokinetic parameters revealed that the AUC and C-max values of d-alpha-lipoic acid were statistically significantly higher than those of l-alpha-lipoic acid (p < 0.05). It was suggested that l-alpha-lipoic acid is more bioavailable than d-alpha-lipoic acid despite individual differences in excretion rate. (C) 2019 Elsevier B.V. All rights reserved.
英文关键词	alpha-Lipoic acid Enantiomer LC/MS/MS Urine
类型	Article
语种	英语
国家	Japan
收录类别	SCI-E
WOS记录号	WOS:000460713700049
WOS类目	Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217271
作者单位	Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, 2-4-41 Ebara, Tokyo 1428501, Japan
推荐引用方式 GB/T 7714	Kobayashi, Yoshiyuki,Ito, Rie,Saito, Koichi. Enantiomeric determination of alpha-lipoic acid in urine by LC/MS/MS[J],2019,166:435-439.
APA	Kobayashi, Yoshiyuki,Ito, Rie,&Saito, Koichi.(2019).Enantiomeric determination of alpha-lipoic acid in urine by LC/MS/MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,166,435-439.
MLA	Kobayashi, Yoshiyuki,et al."Enantiomeric determination of alpha-lipoic acid in urine by LC/MS/MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 166(2019):435-439.