干旱区生态环境知识资源中心(Arid): From H-1 NMR-based non-targeted to LC-MS-based targeted metabolomics strategy for in-depth chemome comparisons among four Cistanche species

Arid

DOI	10.1016/j.jpba.2018.09.013
	From H-1 NMR-based non-targeted to LC-MS-based targeted metabolomics strategy for in-depth chemome comparisons among four Cistanche species
	Liu, Wenjing 1; Song, Qingqing 1; Cao, Yan 1; Xie, Ning 2; Li, Zhiyong 2; Jiang, Yong 3; Zheng, Jian 4; Tu, Pengfei 1; Song, Yuelin 1; Li, Jun 1
通讯作者	Song, Yuelin ; Li, Jun
来源期刊	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN	0731-7085
EISSN	1873-264X
出版年	2019
卷号	162 页码:16-27
英文摘要	The great orthogonality between H-1 NMR spectroscopy and LC-MS implies that their deployments in series could offer an opportunity to gain the qualified molecular markers via comparative metabolomics, and an attempt was made here to propose an integrated strategy namely from H-1 NMR-based non targeted to LC-MS-based targeted metabolomics. In-depth chemome comparisons of Cistanche plants, such as C. deserticola, C. salsa, C. tubulosa, and C. sinensis, that possess dramatic economic and ecological benefits for the arid regions in the northwest China attributing to their dramatic medicinal and edible values, were employed to verify the applicability. H-1 NMR-based non-targeted matabolomics acted as the survey experiment to find those signals offering decisive contributions towards the species discrimination, and the signals were translated to a set of putative identities, eighteen ones in total, through matching with authentic compounds and referring to some accessible databases. Afterwards, an advanced LC-MS platform assembling reversed phase liquid chromatography, hydrophilic interaction liquid chromatography, and tailored multiple reaction monitoring, was introduced to simultaneously quantify those eighteen potential markers in a single analytical run, because those candidates exhibited great polarity span as well as wide content range. Significant species differences occurred amongst their chemome patterns. Echinacoside, acteoside, betaine, mannitol, 6-deoxycatalpol, sucrose, and 8-epi-loganic acid were disclosed as the markers enabling the discrimination of those four species. The findings offered an alternative tool to differentiate Cistanche plants. More importantly, the strategy namely from H-1 NMR-based non-targeted to LC-MS-based targeted metabolomics facilitates the pursuit of molecular markers among analogue plants, and thereby provides a promising choice for in-depth chemome comparison. (C) 2018 Elsevier B.V. All rights reserved.
英文关键词	Chemome comparison Cistanche plants H-1 NMR-based non-targeted metabolomics LC-MS-based targeted metabolomics Species differences
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000449244100003
WOS关键词	TANDEM MASS-SPECTROMETRY ; DIODE-ARRAY DETECTION ; PHENYLETHANOID GLYCOSIDES ; HERBA CISTANCHES ; 1-METHYL-4-PHENYLPYRIDINIUM ION ; QUANTITATIVE-ANALYSIS ; PEUCEDANI RADIX ; IDENTIFICATION ; SALSA ; DISCRIMINATION
WOS类目	Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
来源机构	北京大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/217268
作者单位	1.Beijing Univ Chinese Med, Modern Res Ctr Tradit Chinese Med, Sch Chinese Mat Med, Beijing 100029, Peoples R China; 2.Jiangxi Qingfeng Pharmaceut Co Ltd, State Key Lab Innovat Nat Med & TCM Inject, Ganzhou 341000, Jiangxi, Peoples R China; 3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China; 4.Natl Inst Food & Drug Control, Beijing 100050, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenjing,Song, Qingqing,Cao, Yan,et al. From H-1 NMR-based non-targeted to LC-MS-based targeted metabolomics strategy for in-depth chemome comparisons among four Cistanche species[J]. 北京大学,2019,162:16-27.
APA	Liu, Wenjing.,Song, Qingqing.,Cao, Yan.,Xie, Ning.,Li, Zhiyong.,...&Li, Jun.(2019).From H-1 NMR-based non-targeted to LC-MS-based targeted metabolomics strategy for in-depth chemome comparisons among four Cistanche species.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,162,16-27.
MLA	Liu, Wenjing,et al."From H-1 NMR-based non-targeted to LC-MS-based targeted metabolomics strategy for in-depth chemome comparisons among four Cistanche species".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 162(2019):16-27.