干旱区生态环境知识资源中心(Arid): Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet

Arid

DOI	10.1016/j.jep.2019.111893
	Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet
	El-shiekh, Riham A.1; Al-Mahdy, Dalia A.1; Mouneir, Samar M.2; Hifnawy, Mohamed S.1; Abdel-Sattar, Essam A.1
通讯作者	Al-Mahdy, Dalia A.
来源期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	0378-8741
EISSN	1872-7573
出版年	2019
卷号	238
英文摘要	Ethnopharmacological relevance: Solenostemma argel (Argel) is a desert plant commonly used in Egyptian and Sudanese traditional medicine to suppress appetite, for treatment of diabetes, and as an antispasmodic and anti-inflammatory agent. Previously the anti-diabetic, hypolipidemic and lipase inhibitory activities of Argel were reported in animal studies and in-vitro assays. However, its specific mechanism of action as an anti-obesity agent has not been studied before. Aim of the study: Assessment of the possible anti-obesity effect of Solenostemma argel on diet-induced obesity and elucidation of its mechanism of action, as well as, standardization of the active plant extract. Materials and methods: The ethanolic extract (EtOH-E) and its fractions (CH2Cl2-F: methylene chloride and BuOH-F: n-butanol) were prepared from the aerial parts of S. argel and studied at two dose levels; 200 and 400 mg kg(-1) in a model of high fat diet (HFD) fed rats. The animals (72 Male Wister rats) were assigned into 9 groups: group (i) fed with normal diet and groups (ii-iv) fed with high fat diet (HFD) for 16 weeks and treated with orlistat, EtOH-E, CH2Cl2-F and BuOH-F in the beginning of the 8th week. At the end of the experiment, blood samples were analysed for lipid and liver biomarkers, glucose and insulin levels, as well as, adipokines and inflammatory markers. Liver and adipose tissues were examined histopathologically and their homogenates were used to determine levels of oxidative stress markers and lipogenesis-related genes. Body weight was monitored weekly during the experiment. Results: Our data showed that consumption of S. argel significantly controlled weight gain, attenuated liver steatosis, improved the lipid profile, modulated adipokines activities, increased beta-oxidation gene expression, as well as, decreased the expression of lipogenesis-related genes and ameliorated inflammatory and lipid peroxidation derangement. The ethanolic extract was also standardized using LC-MS analysis for its content of stemmoside C. Conclusions: The current study revealed that S. argel is a promising Egyptian natural drug, rich in pregnane glycosides, and could be considered a new therapeutic candidate targeting obesity.
英文关键词	Diet-induced obesity Solenostemma argel Stemmoside C Body weight gain control Adipokines Lipolysis
类型	Article
语种	英语
国家	Egypt
收录类别	SCI-E
WOS记录号	WOS:000476964300043
WOS关键词	OXIDATIVE STRESS ; ADIPOSE-TISSUE ; 14,15-SECOPREGNANE GLYCOSIDES ; INSULIN-RESISTANCE ; SEED EXTRACT ; FOOD-INTAKE ; WEIGHT-LOSS ; INFLAMMATION ; APPETITE ; PREGNANE
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216984
作者单位	1.Cairo Univ, Dept Pharmacognosy, Fac Pharm, El Kasr El Aini St, Cairo 11562, Egypt; 2.Cairo Univ, Dept Pharmacol, Fac Vet Med, Cairo, Egypt
推荐引用方式 GB/T 7714	El-shiekh, Riham A.,Al-Mahdy, Dalia A.,Mouneir, Samar M.,et al. Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet[J],2019,238.
APA	El-shiekh, Riham A.,Al-Mahdy, Dalia A.,Mouneir, Samar M.,Hifnawy, Mohamed S.,&Abdel-Sattar, Essam A..(2019).Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet.JOURNAL OF ETHNOPHARMACOLOGY,238.
MLA	El-shiekh, Riham A.,et al."Anti-obesity effect of argel (Solenostemma argel) on obese rats fed a high fat diet".JOURNAL OF ETHNOPHARMACOLOGY 238(2019).