干旱区生态环境知识资源中心(Arid): Peptide enrichment by ion-pair solid-phase extraction

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DOI	10.1016/j.jchromb.2019.05.015
	Peptide enrichment by ion-pair solid-phase extraction
	Judak, Peter 1; Polet, Michael 1; Van Eenoo, Peter 1; Benoit, Aurelie 2; Buisson, Corinne 2; Deventer, Koen 1
通讯作者	Judak, Peter
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2019
卷号	1121 页码:89-95
英文摘要	The technique of Solid-Phase Extraction (SPE) is widely used in various fields to concentrate samples and the search for tools to improve recoveries remains of outmost importance. The use of polymer based cartridges has become prevailing in a broad range of fields to enrich peptides from biological matrices. However, the existing SPE protocols are characterized by disparity. Ion-pairing (IP) reagents are commonly used in chromatographic applications, but their combination with SPE is less known. The aim of this study was to evaluate various SPE loading conditions, including the use of IP reagents, to improve the recoveries of nine selected peptide molecules. Control of pH and the use of IP reagents were found to be crucial to improve the enrichment of the peptides, especially cationic peptides, for which an up to ten-fold increase was observed. The practical potential of the presented theoretical findings were verified by employing IP-SPE for the development of an efficient extraction method for the doping relevant peptide Synacthen. The general proof of principle was obtained by analysis of excretion study urine samples and validation was performed with focus on the limit of detection (20 pg/ml) and recovery (37%).
英文关键词	Ion-pairing SPE Peptides Oasis HLB Synacthen Tetracosactide ACTH
类型	Article
语种	英语
国家	Belgium ; France
收录类别	SCI-E
WOS记录号	WOS:000471207200011
WOS关键词	IMMUNOAFFINITY PURIFICATION ; LIQUID-CHROMATOGRAPHY ; HUMAN PLASMA ; URINE ; ADDITIVES ; INSULIN ; QUANTIFICATION ; VALIDATION ; RETENTION ; SYNACHTEN
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216844
作者单位	1.Univ Ghent, Dept Clin Chem Microbiol & Immunol, Doping Control Lab, Technol Pk 30 B, B-9052 Zwijnaarde, Belgium; 2.AFLD, Dept Anal, 143 Ave Roger Salengro, F-92290 Chatenay Malabry, France
推荐引用方式 GB/T 7714	Judak, Peter,Polet, Michael,Van Eenoo, Peter,et al. Peptide enrichment by ion-pair solid-phase extraction[J],2019,1121:89-95.
APA	Judak, Peter,Polet, Michael,Van Eenoo, Peter,Benoit, Aurelie,Buisson, Corinne,&Deventer, Koen.(2019).Peptide enrichment by ion-pair solid-phase extraction.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1121,89-95.
MLA	Judak, Peter,et al."Peptide enrichment by ion-pair solid-phase extraction".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1121(2019):89-95.