Arid
DOI10.1016/j.jchromb.2019.04.029
UPLC-HRMS and NMR applied in the evaluation of solid-phase extraction methods as a rational strategy of dereplication of Phyllanthus spp. aiming at the discovery of cytotoxic metabolites
Souza Silva, Maria Francilene1; Silva, Lorena Mara A.2; Quintela, Amanda Lemos3; dos Santos, Andre Gonzaga4; Nunes Silva, Francisca Aliny1; de Oliveira, Fatima de Cassia E.1; Alves Filho, Elenilson Godoy1; de Brito, Edy Sousa2; Canuto, Kirley Marques2; Pessoa, Claudia1; Zocolo, Guilherme Juliao2
通讯作者Zocolo, Guilherme Juliao
来源期刊JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
EISSN1873-376X
出版年2019
卷号1120页码:51-61
英文摘要The classical approach to drug discovery from natural products (NP's) requires strenuous and complex purification steps for the isolation and structural elucidation. Modern strategies as dereplication aim to accelerate the identification of known compounds present in a crude or partially purified extract. In this work, we investigated the influence of the solid-phase extraction (Oasis, Plexa, and Agilent C18 cartridges with and without organic modifiers) chemical profile obtained by UPLC-QTOF-MS and NMR and cytotoxicities of aqueous extracts from Phyllanthus niruri and P. amarus. Our results showed differences between the SPE cartridges and the mass recovered. P. niruri showed higher mass recovery than P. amarus indicating a higher amount of secondary metabolites. The UPLC-QTOF-MS analysis revealed that P. niruri crude extract presents higher contents of phenolic compounds than P. amarus. According to NMR analysis, P. niruri contained more tyrosine, corilagin, and glycosidic residues while P. amarus, presented higher content of ellagic acid. The different stationary phases, as well as mobile phases for exploratory SPE, enabled the exploitation of the different chemical functionalities within the Phyllanthus species. The SPE (MeOH:H2O 70:30 with C18 cartridges) samples showed greater in vitro cytotoxicity than the crude extracts, with IC50 ranging from 8.01 to 94.92 mu g mL(-1) against the tumor lines tested. The solid phase extraction allowed the concentration of molecules with desirable physicochemical characteristics, which might increase the hit of therapeutically useful substances.
英文关键词Stone break Phyllanthus spp. Cancer Dereplication Cytotoxicity
类型Article
语种英语
国家Brazil
收录类别SCI-E
WOS记录号WOS:000470042300007
WOS关键词PERFORMANCE LIQUID-CHROMATOGRAPHY ; MEDICINAL-PLANT ; NIRURI ; AMARUS ; HEPATITIS ; IDENTIFICATION ; RATS ; SPECTROSCOPY ; ANTITUMOR ; COMPOUND
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/216843
作者单位1.Univ Fed Ceara, NPDM, Rua Coronel Nunes de Mello 1000, BR-60420275 Fortaleza, Ceara, Brazil;
2.Embrapa Agroind Trop, Rua Doutora Sara Mesquita,2270 Pici, BR-60511110 Fortaleza, Ceara, Brazil;
3.Univ Fed Ceara, Dept Analyt & Phys Chem Chem, Ctr Sci, Campus Pici, BR-60455760 Fortaleza, Ceara, Brazil;
4.Sao Paulo State Univ Unesp, Sch Pharmaceut Sci, Dept Nat Principles & Toxicol, Rodovia Araraquara Jau,Km 01, BR-14800903 Araraquara, SP, Brazil
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Souza Silva, Maria Francilene,Silva, Lorena Mara A.,Quintela, Amanda Lemos,et al. UPLC-HRMS and NMR applied in the evaluation of solid-phase extraction methods as a rational strategy of dereplication of Phyllanthus spp. aiming at the discovery of cytotoxic metabolites[J],2019,1120:51-61.
APA Souza Silva, Maria Francilene.,Silva, Lorena Mara A..,Quintela, Amanda Lemos.,dos Santos, Andre Gonzaga.,Nunes Silva, Francisca Aliny.,...&Zocolo, Guilherme Juliao.(2019).UPLC-HRMS and NMR applied in the evaluation of solid-phase extraction methods as a rational strategy of dereplication of Phyllanthus spp. aiming at the discovery of cytotoxic metabolites.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1120,51-61.
MLA Souza Silva, Maria Francilene,et al."UPLC-HRMS and NMR applied in the evaluation of solid-phase extraction methods as a rational strategy of dereplication of Phyllanthus spp. aiming at the discovery of cytotoxic metabolites".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1120(2019):51-61.
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