干旱区生态环境知识资源中心(Arid): Prostaglandins E-2 and F-2 alpha levels in human menstrual fluid by online Solid Phase Extraction coupled to Liquid Chromatography tandem Mass Spectrometry (SPE-LC-MS/MS)

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DOI	10.1016/j.jchromb.2019.01.025
	Prostaglandins E-2 and F-2 alpha levels in human menstrual fluid by online Solid Phase Extraction coupled to Liquid Chromatography tandem Mass Spectrometry (SPE-LC-MS/MS)
	Canzi, Edione F.1,2; Lopes, Bianca Rebelo 2; Robeldo, Thaiane 3; Borra, Ricardo 3; Da Silva, Maria Fatima G. F.2; Oliveira, Regina, V 2; Maia, Beatriz Helena N. S.1; Cass, Quezia B.2
通讯作者	Cass, Quezia B.
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2019
卷号	1109 页码:60-66
英文摘要	This paper reports an online SPE-LC-MS/MS method for the simultaneous quantification of prostaglandins (PGE(2) and PGF(2 alpha)) in menstrual fluid samples. To meet this goal human peripheral serum was used as surrogate matrix. The analytes were trapped on an OASIS HLB cartridge for 3 min, for sample cleanup and enrichment, and then transferred during only 42 s to an HSS T3 C18 analytical column, for separation and analysis. Prostaglandins (PGs) were detected by selected reaction monitoring in negative ion mode, PGE(2) (m/z 351 -> 315) and PGF(2 alpha) (m/z 353 -> 193) using isotope-labeled internal standard (PGE(2)-d(4), m/z 355 -> 319). The concentration linear range was of 10.34-1.034 ng mL(-1) and the lower limit of quantification (LLOQ) was 10.34 ng mL(-1) for both PGs. Validation parameters were successfully assessed according to the European Medicines Agency guideline (EMA), also comprising the FDA normative. The method showed no matrix effect and process efficiency around 100%, in addition to only 15 min of analysis time with lower solvent consumption. The method application was carried out using two menstrual fluid sample groups: control (n = 15) and treatment group (n = 7; samples from women that used Tahiti lemon juice). The PGF(2 alpha) levels were found to be higher in treated group than in control group (p <= 0.05), denoting an effect of the intake of Tahiti lemon juice on the menstrual inflammatory process. The on-line method herein reported could be useful for the analysis of PGs from large research studies.
英文关键词	Prostaglandins PGE(2) and PGF(2 alpha) Online SPE LC-MS/MS Menstrual fluid
类型	Article
语种	英语
国家	Brazil
收录类别	SCI-E
WOS记录号	WOS:000461411800008
WOS关键词	SURROGATE MATRIX ; QUANTIFICATION ; PLASMA ; EICOSANOIDS ; MECHANISMS ; LEUKOCYTES
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216841
作者单位	1.Univ Fed Parana UFPR, Dept Quim, Ctr Politecn, BR-81531990 Curitiba, Parana, Brazil; 2.Univ Fed Sao Carlos, Dept Quim, Rodovia Washington Luis,Km 235,SP-310, BR-13565905 Sao Carlos, SP, Brazil; 3.Univ Fed Sao Carlos, Dept Genet & Evolucao, Rodovia Washington Luis,Km 235,SP-310, BR-13565905 Sao Carlos, SP, Brazil
推荐引用方式 GB/T 7714	Canzi, Edione F.,Lopes, Bianca Rebelo,Robeldo, Thaiane,et al. Prostaglandins E-2 and F-2 alpha levels in human menstrual fluid by online Solid Phase Extraction coupled to Liquid Chromatography tandem Mass Spectrometry (SPE-LC-MS/MS)[J],2019,1109:60-66.
APA	Canzi, Edione F..,Lopes, Bianca Rebelo.,Robeldo, Thaiane.,Borra, Ricardo.,Da Silva, Maria Fatima G. F..,...&Cass, Quezia B..(2019).Prostaglandins E-2 and F-2 alpha levels in human menstrual fluid by online Solid Phase Extraction coupled to Liquid Chromatography tandem Mass Spectrometry (SPE-LC-MS/MS).JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1109,60-66.
MLA	Canzi, Edione F.,et al."Prostaglandins E-2 and F-2 alpha levels in human menstrual fluid by online Solid Phase Extraction coupled to Liquid Chromatography tandem Mass Spectrometry (SPE-LC-MS/MS)".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1109(2019):60-66.