干旱区生态环境知识资源中心(Arid): Total determination of triclabendazole and its metabolites in bovine tissues using liquid chromatography-tandem mass spectrometry

Arid

DOI	10.1016/j.jchromb.2019.01.021
	Total determination of triclabendazole and its metabolites in bovine tissues using liquid chromatography-tandem mass spectrometry
	Kikuchi, Hiroyuki; Sakai, Takatoshi; Okura, Tomoko; Nemoto, Satoru; Akiyama, Hiroshi
通讯作者	Kikuchi, Hiroyuki
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2019
卷号	1109 页码:54-59
英文摘要	A reliable LC-MS/MS analytical method for the determination of residual triclabendazole and its principal metabolites (triclabendazole sulfoxide, triclabendazole sulfone and keto-triclabendazole) in bovine tissues was developed, in which triclabendazole and its metabolites are oxidized to keto-triclabendazole as a marker residue. The method involves sample digestion with hot sodium hydroxide, thus releasing the bound residues of various triclabendazole metabolites in bovine tissues. The target compounds are extracted from the digest mixture with ethyl acetate, defatted by liquid-liquid partitioning using n-hexane and acetonitrile, then oxidized with hydrogen peroxide in a mixture of ethanol and acetic acid. The reaction mixture is cleaned up using a strong cation exchange cartridge (Oasis MCX) and the analytes are quantified using LC-MS/MS. The optimal conditions for the complete oxidation of triclabendazole and its metabolites to keto-triclabendazole are an incubation time of 16 h and a temperature of 90 degrees C. The developed method was evaluated using three bovine samples: muscle, fat, and liver. Samples were spiked with triclabendazole and its principal metabolites at 0.01 mg/kg and at the Japanese Maximum Residue Limits (MRLs) established for each sample. The validation results show excellent recoveries (81-102%) and precision (< 10%) for all target compounds. The limit of quantification (S/N >= 10) of the developed method is 0.01 mg/kg. These results suggest the developed method is applicable to quantifying residual triclabendazole in bovine tissues in compliance with the MRLs established by the Codex Alimentarius and EU and Japanese regulations, and thus the proposed method will be a useful tool for the regulatory monitoring of residual triclabendazole and its metabolites.
英文关键词	Triclabendazole Triclabendazole sulfoxide Triclabendazole sulfone Keto-triclabendazole LC-MS/MS
类型	Article
语种	英语
国家	Japan
收录类别	SCI-E
WOS记录号	WOS:000461411800007
WOS关键词	MILK ; RESIDUES ; LIVER ; BENZIMIDAZOLES ; EXTRACTION
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216839
作者单位	Natl Inst Hlth Sci, Div Foods, Kawasaki Ku, 3-25-6 Tonomachi, Kawasaki, Kanagawa 2109501, Japan
推荐引用方式 GB/T 7714	Kikuchi, Hiroyuki,Sakai, Takatoshi,Okura, Tomoko,et al. Total determination of triclabendazole and its metabolites in bovine tissues using liquid chromatography-tandem mass spectrometry[J],2019,1109:54-59.
APA	Kikuchi, Hiroyuki,Sakai, Takatoshi,Okura, Tomoko,Nemoto, Satoru,&Akiyama, Hiroshi.(2019).Total determination of triclabendazole and its metabolites in bovine tissues using liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1109,54-59.
MLA	Kikuchi, Hiroyuki,et al."Total determination of triclabendazole and its metabolites in bovine tissues using liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1109(2019):54-59.