干旱区生态环境知识资源中心(Arid): Identification and mapping of brain natriuretic peptide in the normal ventricular myocardium of a desert-dwelling mammalian model, the camel (Camelus dromedarius): Immunohistochemical and ultrastructural study

Arid

DOI	10.1002/jcp.27126
	Identification and mapping of brain natriuretic peptide in the normal ventricular myocardium of a desert-dwelling mammalian model, the camel (Camelus dromedarius): Immunohistochemical and ultrastructural study
	Osman, Abdel-Hamid K.1; Minamino, Naoto 2; Marei, Hany E.3
通讯作者	Osman, Abdel-Hamid K.
来源期刊	JOURNAL OF CELLULAR PHYSIOLOGY
ISSN	0021-9541
EISSN	1097-4652
出版年	2019
卷号	234 期号:3 页码:3067-3077
英文摘要	Brain natriuretic peptide (BNP) is mainly produced in the ventricular myocardium, where it is released into the circulation, producing rapid volume decrease by diuresis, natriuresis, and water shift into the extracellular space, and vasodilation. The dromedary camel, a mammalian model of the desert nomads, lives under unfavorable physiological stresses during thirst, starvation, desiccation, and hot climate, thus has a special demand for water homeostasis. The present studies characterized BNP in the ventricular myocardium of healthy camels, immunohistochemically with a specific antibody, and ultrastructurally identified the endocrine property of the cardiomyocytes and Purkinje fibers. The paranuclear, granular, immunoreactive material was not restricted to the cardiomyocytes, as it was also visible in the Purkinje fibers and their associated nerve varicosities. The intensity of immunoreactive BNP showed a transmural gradient from the subepicardium to the myocardium. Intense immunoreactivity was also noted among the perivascular cardiomyocytes. At the electron microscopic level, specific granules were demonstrated in the paranuclear cytosol of cardiomyocytes and Purkinje fibers. The current study provides the first immunohistochemical localization pattern of BNP in the camel myocardium and suggests a relationship between the intense subepicardial BNP-immunoexpression and a possible translocation of the active hormone to the pericardial fluid for further paracrine actions on the heart and its coronaries.
英文关键词	BNP camel cardiomyocytes heart immunohistochemistry myoendocrine cells natriuretic peptides Purkinje fibers ultrastructure
类型	Article
语种	英语
国家	Egypt ; Japan ; Qatar
收录类别	SCI-E
WOS记录号	WOS:000451618500095
WOS关键词	CARDIAC MAST-CELLS ; IMMUNOREACTIVE ATRIAL ; PERICARDIAL FLUID ; PURKINJE-FIBERS ; HEART ; LOCALIZATION ; EXPRESSION ; HYPERTROPHY ; SYSTEM ; ANP
WOS类目	Cell Biology ; Physiology
WOS研究方向	Cell Biology ; Physiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/216832
作者单位	1.Suez Canal Univ, Fac Vet Med, Dept Cytol & Histol, Ismailia 41511, Egypt; 2.Natl Cerebral & Cardiovasc Ctr, Res Inst, Dept Mol Pharmacol, Suita, Osaka, Japan; 3.Qatar Univ, Biomed Res Ctr, Dept Biomed Sci, Doha, Qatar
推荐引用方式 GB/T 7714	Osman, Abdel-Hamid K.,Minamino, Naoto,Marei, Hany E.. Identification and mapping of brain natriuretic peptide in the normal ventricular myocardium of a desert-dwelling mammalian model, the camel (Camelus dromedarius): Immunohistochemical and ultrastructural study[J],2019,234(3):3067-3077.
APA	Osman, Abdel-Hamid K.,Minamino, Naoto,&Marei, Hany E..(2019).Identification and mapping of brain natriuretic peptide in the normal ventricular myocardium of a desert-dwelling mammalian model, the camel (Camelus dromedarius): Immunohistochemical and ultrastructural study.JOURNAL OF CELLULAR PHYSIOLOGY,234(3),3067-3077.
MLA	Osman, Abdel-Hamid K.,et al."Identification and mapping of brain natriuretic peptide in the normal ventricular myocardium of a desert-dwelling mammalian model, the camel (Camelus dromedarius): Immunohistochemical and ultrastructural study".JOURNAL OF CELLULAR PHYSIOLOGY 234.3(2019):3067-3077.